buffers

Supplier: Merck Millipore (Novagen)

BugBuster® Protein Extraction reagent is formulated for the gentle disruption of the cell wall of E. coli, liberating soluble protein. It provides a simple, rapid, low-cost alternative to mechanical methods such as French Press or sonication for releasing expressed target protein in preparation for purification or other applications. The formulation utilises a detergent mix that is capable of cell wall perforation without denaturing soluble protein.

Supplier: Corning

Sodium Dodecyl Sulfate (SDS) is an anionic detergent with applications in SDS-PAGE protein analysis and nucleic acid purification. It is a wetting agent that is effective in both acid and alkaline solutions, and is most often used as a protein and lipid solubilization reagent. SDS is also a powerful protein denaturant. This 10% solution is prepared in 18 megaohm water and is 0.2µm filtered.

Supplier: G-Biosciences

Solution developed to unmask antigens in paraffin embedded tissue sections. 

Supplier: Corning

EDTA (ethylenediaminetetraacetic acid) is a polyamino carboxylic acid. It is a colorless, water-soluble solid and is useful as a chelating agent due to its ability to "sequester" metal ions such as Ca2+ and Fe3+.  These cations are responsible for various undesirable effects in molecular biological and cell culture processes. After being bound by EDTA, these metal ions remain in the solution but exhibit diminished reactivity.  The use of EDTA therefore allows for greater control over many experiments.

Supplier: G-Biosciences

ACD (Acid Citrate Dextrose) Solution A and ACD (Acid Citrate Dextrose) Solution B are anticoagulants for whole blood and also act as acidifying agents to platelet poor plasma.

Supplier: WTW

Ion strength adjustment solutions have multiple tasks: TISAB for fluoride measurements does not only adjust the pH value, by also decomplexes the fluoride-containing complex compounds. Other ion strength adjustment solutions ensure an adapted pH value as well as the addition of a suitable conducting salt to correctly capture the concentration.

Supplier: REAGECON

Buffer solution, pH value: 4,00, ±0,01, Reference temperature: 25 °C, Plastic bottle

Supplier: Merck Millipore (Calbiochem‎)

Use of tetraethylammonium (TEA) and Tris loading for blocking TRPM7 channels in intact cells.: This study highlights the application of TRIS Hydrochloride in blocking TRPM7 channels, which is significant for cell signaling and ion channel regulation in pharmacological research (Holderby & Kozak, 2024).

Supplier: Merck Millipore (Novagen)

BugBuster® HT Protein Extraction Reagent combines BugBuster® Protein Extraction Reagent and Benzonase® Nuclease in one convenient reagent. BugBuster® HT eliminates common bioprocessing problems resulting from traditional cell lysis procedures. Soluble proteins are gently extracted from E. coli without exposure to heat or oxidative damage and viscosity is eliminated by nucleic acid digestion in a single step. The resulting protein extract can easily be fractionated by conventional purification techniques. BugBuster HT is ideally suited for application in high throughput protein purifications.

Supplier: REAGECON

Colour coded pH buffer solutions for the calibration of pH meters.

Supplier: EUROCLONE

HEPES buffer solution (1 mol/l)

Supplier: MP Biomedicals

Cell culture buffer, Hank's balanced salt solution, modified (1X)

Supplier: EUROCLONE

HEPES buffer solution (1 mol/l)

Supplier: REAGECON

This range of Reagecon pH Buffers are sodium tetraborate (Borax) free. They are not subject to classification by EU regulatory authorities as toxic to reproduction under CLP category 1B. Similar in approach to the existing range of Reagecon Mercury Free Buffers, these Buffer Solutions have been developed to enhance user choice and allay safety concerns, without any impact on fitness for purpose or product performance.

Supplier: WTW

Adapted to different electrode types for calibration and maintenance.

Supplier: MP Biomedicals

Hanks' balanced salts solution is used to maintain the pH and osmotic balance in the medium and to provide the cells with water and essential inorganic ions.