buffers

Resisting pH changes, buffers protect samples when acids or alkali is added. Powder and liquid forms efficiently track proteins during electrophoresis isolation and separation. The salty buffers can also be used to calibrate and standardize pH meters for accurate readings. Blocking substances stop antibody attachment to membrane free sites that would produce an interfering background. In various concentrations to fit any need, the buffers may come in liquids or dehydrated formats.

Y-PER™ plus Yeast Protein Extraction Reagent, Dialysable

Supplier: Thermo Fisher Scientific

This extraction reagent is formulated to effectively lyse yeast and bacterial cells to extract and solubilise proteins for analysis.

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HyClone™ Earle's 1X Balanced Salt Solution (EBSS): Solution

Supplier: HyClone products (Cytiva)

Balanced salt solution for cell culture applications.

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IPG buffers

Supplier: Cytiva

IPG buffers are ampholyte-containing buffer concentrates specifically formulated for use with Immobiline™ DryStrip gels for isoelectric focusing, run as the first dimension of 2-D electrophoresis or as a separate application. Using Immobiline DryStrip gels together with matching IPG buffer gives high sample loading capacity, improves resolution, and increases the probability of detecting low abundance proteins.

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Y-PER™ Yeast Protein Extraction Reagent

Supplier: Thermo Fisher Scientific

This reagent uses a mild detergent lysis procedure to efficiently release functionally active solubilised proteins.

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$Subcellular protein fractionation kit for tissues$

Subcellular protein fractionation kit for tissues

Supplier: Thermo Fisher Scientific

This kit is ideal for cell fractionation and enrichment of proteins from five different cellular compartments within two hours.

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Mammalian protein extract reagents, M-PER™

Supplier: Thermo Fisher Scientific

The M-PER Mammalian Protein Extraction Reagent is designed to provide highly efficient total protein extraction from cultured mammalian cells.

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Buffers, TBS, 20X solution, pH 7,4, molecular biology grade, ultrapure

Supplier: Thermo Fisher Scientific

Used in Western blotting protocols.

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Cesium chloride - lanthanum chloride buffer, for flame AAS, TraceSELECT™, Fluka™

Supplier: Honeywell Chemicals

TraceSELECT™, for flame atomic absorption spectrometry.

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Subcellular Protein Fractionation Kit for Cultured Cells

Supplier: Thermo Fisher Scientific

This kit is ideal for segregation and enrichment of proteins from five different cellular compartments.

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CSPL buffer

Supplier: OMEGA BIO-TEK

Component of the Mag-Bind® plant DNA 96 plus kit.

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Ab buffer kit

Supplier: Cytiva

Ab buffer kit contains prepared buffer concentrates for binding, washing, and elution of IgG according to recommended protocols.

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HEPES Buffer, 1 M Sterile, Cell Culture Grade, MP Biomedicals

Supplier: MP Biomedicals

HEPES (N-2-hydroxyethylpiperazine-N-2-ethane sulfonic acid) is a general purpose zwitterionic organic chemical buffering agent which does not bind magnesium, calcium, manganese(II) or copper (II) ions.

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Mini flip-top vial with 9 ml Letheen Broth

Supplier: Neogen

The 3M™ Mini Flip-Top Vial with 9 ml Letheen Broth is a leak-resistant plastic cylinder with a flip-open lid that seals securely for safe shipping and handling.

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PBS, 10X solution, molecular biology grade, ultrapure

Supplier: Thermo Fisher Scientific

Washing buffer, PBS, 10X solution, molecular biology grade, ultrapure

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Protein A/G HP SpinTrap™ Buffer Kit

Supplier: Cytiva

Protein A/G HP SpinTrap™ Buffer kit is optimised for standard affinity-based enrichment/immunoprecipitation according to the protocol provided with protein A HP SpinTrap™, protein G HP SpinTrap™, and Ab SpinTrap™ columns.