buffers

Resisting pH changes, buffers protect samples when acids or alkali is added. Powder and liquid forms efficiently track proteins during electrophoresis isolation and separation. The salty buffers can also be used to calibrate and standardize pH meters for accurate readings. Blocking substances stop antibody attachment to membrane free sites that would produce an interfering background. In various concentrations to fit any need, the buffers may come in liquids or dehydrated formats.

Nuclear Extraction Kit, Abcam

Supplier: Abcam

Nuclear extraction kit provides a simple and selective method for nuclear protein extraction and fractionation from cells or tissue samples in just 1 hour. This kit includes all necessary reagents to obtain high yields of nuclear proteins suitable for various downstream applications. - Cited in over 255 publications - High retention of enzyme activity – contains 80% less salt than conventional kits and no SDS, allowing better enzyme activity retention - Suitable for western blotting, protein-DNA binding assays, nuclear enzyme assays, and more - Fast, simple procedure that ensures optimized nuclear protein isolation

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Nuclear (Nucleic Acid-Free) Extraction Kit, Abcam

Supplier: Abcam

Nuclear (Nucleic Acid-Free) Extraction Kit (ab113477) provides a simple and selective method for extracting nuclear proteins (nucleic-acid free) in just 1 hour for a variety of applications such as western blotting, protein-DNA binding assays, or any other procedures requiring optimized nucleic acid-free nuclear proteins.

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Buffer, TRIS solution pH 7 (1 mol/L)

Supplier: Cayman Chemical

Cloning Buffer, TRIS buffer solution pH 7 (1 mol/L)

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Buffer, TRIS solution pH 7,5 (1 mol/L)

Supplier: Cayman Chemical

Cloning Buffer, TRIS buffer solution pH 7,5 (1 mol/l)

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Buffer, TRIS solution pH 8 (1 mol/L)

Supplier: Cayman Chemical

Cloning Buffer, TRIS buffer solution pH 8 (1 mol/L)

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Buffers, sodium phosphate buffer solutions (0,5 mol/L)

Supplier: Cayman Chemical

Sodium phosphate stock solution (0,5 mol/L), pH value: 6,0

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Buffers, potassium phosphate buffer (0,5 mol/L) solutions

Supplier: Cayman Chemical

Potassium phosphate buffer solution (0,5 mol/l), pH value: 6,0

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Buffer, HEPES solution (1 mol/L)

Supplier: Cayman Chemical

HEPES buffer solution (1 mol/l)

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Buffer, EDTA buffer solution pH 8,0 (0,5 mol/L)

Supplier: Cayman Chemical

Cloning Buffer, EDTA buffer solution pH 8,0 (0,5 mol/l)

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Buffer, TBS solution 10X concentrate (TRIS buffered saline) with phenol red, pH 7,4

Supplier: Cayman Chemical

250 mM Tris-base, pH 7,4, 1,37 M sodium chloride, containing 27 mM KCl, 0,4 mM phenol red.

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Buffer, sodium acetate buffer solution pH 5,2 (3 mol/L)

Supplier: Cayman Chemical

Cloning Buffer, Sodium acetate buffer solution pH 5,2 (3 mol/l)

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Buffer, TBS solution 10X concentrate (TRIS buffered saline) pH 7,4

Supplier: Cayman Chemical

Formulation: Concentrated TBS (250 mM Tris-base; pH 7,4; 1,37 M sodium chloride and 27 mM KCl).

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10X Blocking Buffer, Abcam

Supplier: Abcam

10X blocking buffer (pH 7.5) for serum-free blocking of non-specific antibody binding. - Cited in over 30 publications - Suitable for ELISA, in-cell ELISA, ICC/IF, western blot (WB) - 100% casein blocking as preservative

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Blocking buffers

Supplier: Rockland Immunochemicals

A blocking buffer specifically designed for western blotting using fluorochrome conjugated antibodies. Blocking buffer was prepared using ultra pure reagents dissolved in pharmaceutical grade water (WFI) and consists of a proprietary protein formulation in tris buffered saline at pH 7,6 with thimerisol added as an antimicrobial agent.