buffers

Resisting pH changes, buffers protect samples when acids or alkali is added. Powder and liquid forms efficiently track proteins during electrophoresis isolation and separation. The salty buffers can also be used to calibrate and standardize pH meters for accurate readings. Blocking substances stop antibody attachment to membrane free sites that would produce an interfering background. In various concentrations to fit any need, the buffers may come in liquids or dehydrated formats.

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Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0), Abcam

Supplier: Abcam

Antigen retrieval 100X tris-EDTA buffer (pH 9.0) enables target retrieval in formalin-fixed, paraffin-embedded tissue sections in one step. - Cited in over 20 publications - Contains 5% tris, 5% tetrasodium ethylene diamine tetraacetate, 1.5% 2-butoxyethanol - Optimized for tris-EDTA buffer (pH 9.0) treated antibodies - Contains detergent for paraffin emulsification

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Antigen Retrieval Buffer (100X Citrate Buffer), Abcam

Supplier: Abcam

Antigen retrieval 100X citrate buffer (pH 5.1 - 5.5) is a special solution that enables rehydration and antigen retrieval to be performed in formalin-fixed, paraffin-embedded tissue sections with one step. - Cited in over 25 publications - Contains citrate buffer, 1.5% 2-butoxyethanol, 1% hydrochloric acid - Optimized for citrate buffer-treated antibodies - Contains detergent for paraffin emulsification

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Buffer, sodium acetate buffer solution pH 5,2 (3 mol/L)

Supplier: Thermo Fisher Scientific

Cloning Buffer, Sodium acetate buffer solution pH 5,2 (3 mol/l)

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25x PBS Buffer pH 7.6, Abcam

Supplier: Abcam

In order to provide this product at 25X, the salts in solution are at a very high concentration.

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10x Citrate Buffer pH 6.0, Abcam

Supplier: Abcam

Citrate buffer pH 6.0 for heat-induced antigen retrieval (HIER) during IHC.

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10x EDTA Buffer pH 8.0, Abcam

Supplier: Abcam

EDTA buffer pH 8.0 for heat-induced antigen retrieval (HIER) during IHC (ab64216).

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Protein Block, Abcam

Supplier: Abcam

Protein block is a serum-free blocking buffer designed to reduce non-specific background staining in IHC, ELISA, and western blot (WB). - Cited in over 90 publications - No need to match species for secondary antibodies, reducing animal serum handling - Ready-to-use – no mixing or dilution required

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100x Citrate Buffer pH 6.0, Abcam

Supplier: Abcam

Citrate buffer pH 6.0 for heat-induced antigen retrieval (HIER) during IHC (ab64236).

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Antigen Retrieval Buffer (100X EDTA Buffer, pH 8.0), Abcam

Supplier: Abcam

Antigen Retrieval Buffer (100X EDTA Buffer, pH 8.0) is a special solution that enables rehydrate and target retrieval in formalin-fixed, paraffin-embedded tissue sections in one step.

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Antigen Retrieval Buffer (100X Tris Buffer, pH 10.0), Abcam

Supplier: Abcam

Antigen Retrieval Buffer (100X Tris Buffer, pH 10.0) is a special solution that enables rehydrate and target retrieval in formalin-fixed, paraffin-embedded tissue sections in one step.

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Blocking buffer, fish gelatin

Supplier: Biotium

Fish gelatin is commonly used in blocking solutions to minimise background noise in immunodetection procedures such as Western Blotting and immunostaining. Fish gelatin can be used to block non-specific binding sites on positively charged nylon or PVDF membranes or for blocking and dilution of antibodies during immunostaining procedures. Unlike BSA or milk, fish gelatin does not contain IgG or serum proteins that could cross-react with mammalian antibodies.

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G-Biosciences 10X Concentrated TE Buffers (Tris-EDTA Buffer)

Supplier: G-Biosciences

The tris buffering agent and EDTA metal chelating properties help protect DNA and RNA.

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PBS tablets

Supplier: PanReac AppliChem

Tablets for preparation of 100 ml phosphate buffered saline (PBS).

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Buffer, TBE solution 5X concentrate (TRIS-borate-EDTA buffer)

Supplier: Biotium

Running Buffer, TBE buffer solution 5X concentrate (TRIS-borate-EDTA buffer)

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Gram Negative lysis buffer

Supplier: G-Biosciences

Gram-negative lysis buffer is an extraction buffer for soluble proteins from Gram-negative bacteria. It is a proprietary improvement on the lysozyme based lysis in combination with various salts and agents, which allows extraction of soluble proteins. This buffer has been tested for use with several widely used bacteria including E.coli strains.

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Buffer, PBS tablets

Supplier: PanReac AppliChem

PBS Tablets for preparation of 200 ml phosphate buffered saline (PBS). Composition of 140 mM sodium chloride, 2,7 mM potassium chloride and 10 mM phosphate.