buffers

Resisting pH changes, buffers protect samples when acids or alkali is added. Powder and liquid forms efficiently track proteins during electrophoresis isolation and separation. The salty buffers can also be used to calibrate and standardize pH meters for accurate readings. Blocking substances stop antibody attachment to membrane free sites that would produce an interfering background. In various concentrations to fit any need, the buffers may come in liquids or dehydrated formats.

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VWR®, TE (Tris-EDTA) buffer, pH 8,0 for biotechnology

Supplier: VWR Chemicals

Ready to use Tris-EDTA buffer.

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Buffer solution pH 4, citrate buffer

Supplier: Thermo Fisher Scientific

pH Buffer solution, Citrate, pH value: 4,00, ±0,02, Colourless

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Buffers, concentrated solutions, Titrisol®, Supelco®

Supplier: Merck

Titrisol® buffer concentrate for 500 ml buffer solution [20 °C].

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Buffer, TRIS solution pH 7,5 (1 mol/L)

Supplier: PanReac AppliChem

Cloning Buffer, TRIS buffer solution pH 7,5 (1 mol/l)

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Permeabilisation buffer

Supplier: Biotium

Ready-to-use permeabilisation buffer for intracellular immunofluorescence staining for microscopy or flow cytometry.

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Electrophoresis running buffers

Supplier: G-Biosciences

There are many electrophoresis running buffers including: TAE running buffer (50×), TBE running buffer (10×), TBE-urea sample buffer, MOPS running buffer (10×), and MOPS/ SDS running buffer (20×). These include the SDS-PAGE sample loading buffer, SDS-PAGE running buffer, native sample loading buffer, tris glycine native gel running buffer, tricine sample buffer, tris-tricine, tris-tricine-SDS, MES SDS running buffer, MOPS/ SDS running buffer, IEF anode buffer, IEF cathode buffer, zymogram sample buffer, zymogram renature buffer, zymogram development buffer and the discontinuous buffer system.

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Buffer, TRIS solution pH 8 (1 mol/L)

Supplier: PanReac AppliChem

Cloning Buffer, TRIS buffer solution (1 mol/l)

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Buffer solution pH 5, Acetate Buffer

Supplier: Thermo Fisher Scientific

Acetate buffer, Contains 0.7 ml glacial acetic acid and 0.6 g potassium hydroxide per litre., pH value: 4,98 - 5,02 (20 °C)

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Blocking buffers

Supplier: Rockland Immunochemicals

A blocking buffer specifically designed for western blotting using fluorochrome conjugated antibodies. Blocking buffer was prepared using ultra pure reagents dissolved in pharmaceutical grade water (WFI) and consists of a proprietary protein formulation in tris buffered saline at pH 7,6 with thimerisol added as an antimicrobial agent.

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Dry buffer packs, JAW™

Supplier: G-Biosciences

Premixed buffer powders that simply require adding water to generate a ready-to-use buffer.

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Phosphate Buffer Solution

Supplier: SIGMA ALDRICH MICROSCOPY

Contains sodium and potassium phosphates. Intended for use in the Sigma Butyrate Esterase Procedure, No. 181B.

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Blocking buffers, FirstChoice™

Supplier: G-Biosciences

FirstChoice™ Blocking Buffer is ideal as a first choice for optimization of new assays and systems, or when determining the optimal blocking buffer for elimination of non specific binding sites in ELISA, blotting, immunohistochemistry and other applications. Buffers are conveniently supplied in widely used TBS (Tris-buffered saline at pH 7,5) and PBS (phosphate-buffered saline at pH 7,5) buffers as well as in separate formulations containing Tween® 20 for improving blocking efficiencies.

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Instant Buffers (acc. to Soerensen)

Supplier: TCI

Instant Buffer S70, (acc. to Soerensen), pH value: 7.0

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Phosphate Buffered Saline

Supplier: THERMO FISHER DIAGNOSTICS

Cell culture buffer, Phosphate buffered saline tablets

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Gram Negative lysis buffer

Supplier: G-Biosciences

Gram-negative lysis buffer is an extraction buffer for soluble proteins from Gram-negative bacteria. It is a proprietary improvement on the lysozyme based lysis in combination with various salts and agents, which allows extraction of soluble proteins. This buffer has been tested for use with several widely used bacteria including E.coli strains.