"USLP070318BIOP00127"

Supplier: MP Biomedicals

Set consists of 100 mM aqueous solutions of dATP, dCTP, dGTP and dTTP each supplied in a separate vial.

Supplier: G-Biosciences

Set consists of 100 mM aqueous solutions of dATP, dCTP, dGTP and dTTP each supplied in a separate vial. Also available separately.

Supplier: Cytiva

High purity dNTPs for amplification, dideoxy sequencing, labelling, mutagenesis, cDNA synthesis, and expression profiling.

Supplier: Cytiva

Easy to use, time saving solutions for in vitro transcription or for reactions requiring rNTPs of high purity.

Supplier: OMEGA BIO-TEK

The E.Z.N.A.® RNALock Stabilizer Reagent is an innovative reagent which rapidly lyses cells and tissues to inactivate RNases at room temperature. Simply submerge the samples, such as cell cultures, animal tissues, or white blood cells into the RNALock Stabilizer Reagent. The intact cellular RNA will be preserved even at elevated temperature. Samples can be stored in this reagent in excess of 12 months at −20 °C.

Supplier: Quantabio

PerfeCTa® Multiplex qPCR SuperMix, low ROX is a 2X concentrated, ready to use reaction cocktail that contains all components, except primers, probe(s), and template for real time qPCR on Applied Biosystems 7500 or Stratagene MX series of real time PCR systems.

Supplier: Quantabio

The 10 mM dNTP mix is a solution of high purity deoxyribonucleoside triphosphates that has been functionally qualified for real-time quantitative PCR (qPCR). It is suitable for use in conventional end-point PCR, real time qPCR, first-strand cDNA synthesis, as well as other applications that require dNTP as substrate. The use of qPCR certified dNTPs is a simple but important measure to ensure consistent assay performance.

Supplier: Quantabio

AccuStart™ II PCR SuperMix is a 2X concentrated, ready-to-use reaction cocktail for routine PCR amplification of DNA fragments up to 4 kb. It contains all components, except primers and template. AccuStart™ II PCR SuperMix simplifies reaction assembly, improves assay reproducibility, and reduces the risk of contamination. A key component is AccuStart™ II Taq DNA polymerase which contains monoclonal antibodies that bind to the polymerase and keep it inactive prior to the initial PCR denaturation step. Upon heat activation (1 minute at 94 °C), the antibodies denature irreversibly, releasing fully active, unmodified Taq DNA polymerase. This enables specific and efficient primer extension with the convenience of room temperature reaction assembly.

Supplier: Quantabio

Tämä SuperMix on 2X käyttövalmis reaktioseos reaaliaikaiseen qPCR:ään. Se sisältää kaikki komponentit alukkeita, koettimia ja templaattia lukuun ottamatta. Järjestelmää eivät rajoita perinteisten PCR-reaktiosekoitteiden amplifikaatiorajoitukset, ja jopa viiden kohdesekvenssin amplifikaatio samassa putkessa on mahdollista.Korkean kopioluvun templaattien aiheuttama alhaisen kopioluvun amplimeerien suppressio on tunnettu moninkertaisen PCR:n ongelma, joka voi vääristää tai peittää alhaisen kopioluvun kohdesekvenssien ilmenemisen ja kvantifikaation. PerfeCTa MultiPlex qPCR SuperMix -seoksella on laaja dynaaminen alue ja sen herkkyys moninkertaisessa qPCR:ssä vastaa herkkyyttä yksittäiskoetin qPCR:ssä eikä alukepitoisuuksia tarvitse rajoittaa tai vaihdella.

Supplier: G-Biosciences

Taq DNA Polymerase is a highly thermostable recombinant DNA polymerase derived from the thermophile Thermus aquaticus. The molecular weight of the recombinant protein is 94 kD. The Taq Polymerase is able to amplify DNA up to 5 kb with an elongation velocity of 0,9 to 1,2 kb/min at 70 to 75 °C. The error rate of this Taq Polymerase is ~2,2×10⁻⁵ nucleotide⁻¹ cycle⁻¹. Taq DNA polymerase catalyses the 5’→3’ synthesis of DNA. The enzyme has no detectable 3’→5’ proofreading exonuclease activity, and possesses low 5’→3’ exonuclease activity, which results in a 3’-dA overhang on the PCR product

Supplier: Quantabio

PerfeCTa® qPCR ToughMix® on 2x käyttövalmis reaktioseos DNA-templaattien PCR-monistukseen. Useat tunnetut PCR-inhibiittorit, joita on usein ympäristöstä otetuissa käsittelemättömissä näytteissä, kasvikudoksissa tai eläinkudoksissa, eivät vaikuta seokseen. Kyseessä on monipuolinen ja kestävä reaaliaikaiseen qPCR-ajoon tarkoitettu reagenssi, jonka tuottama PCR-reaktio on herkkä ja tehokas monenlaisilla fluoresoivilla koettimilla, esimerkiksi TaqMan®-hydrolyysikoettimilla. PerfeCTa® qPCR ToughMix® -seoksessa on kaikki tarvittavat reaktiokomponentit, paitsi alukkeet, koettimet ja DNA-templaatit. AccuVue™-väriaineen vaaleansininen väri helpottaa reaktion havainnointia valkoisilla tai läpinäkyvillä levyillä ja vähentää pipetointi- tai sekoitusvirheitä. Väriaine ei häiritse qPCR-reaktiota tai vaikuta tuotteen stabiiliuteen.

Supplier: OMEGA BIO-TEK

Mag-Bind® Viral DNA/RNA kit is designed for the rapid and reliable isolation of viral RNA and viral DNA from whole blood, serum, plasma, saliva and other body fluids. The Mag-Bind® magnetic beads technology enables purification of high quality nucleic acids that is free of proteins, nucleases, and other impurities. In addition to easily being adapted with automated systems, this procedure can also be scaled up or down, allowing for the purification from various amounts of starting materials. The purified nucleic acid is ready for direct use in downstream applications, such as amplification or other enzymatic reactions.