"Staining Solutions"

Supplier: Thermo Fisher Scientific

Thermo Scientific Pierce zinc reversible stain kit provides rapid and highly sensitive detection of protein in polyacrylamide gels immediately after electrophoresis as well as erasability for subsequent transfer or band extraction.

Supplier: Simport Scientific

StainTray M922 is a high-capacity slide staining system.

Supplier: RAL

The spermogram helps assess the percentage of dead and living spermatozoa. Only dead spermatozoa stain pink by Eosin. Living spermatozoa remain colourless, except their outlines stain dark blue by Nigrosin.

Supplier: SIGMA ALDRICH MICROSCOPY

Oil Blue N, Dye content 96% (Solvent Blue 14).

Supplier: Cayman Chemical

Cell-based working solution prepared by dilution of the Nile red stock solution 1:1000 in the assay buffer.

Supplier: SIGMA ALDRICH MICROSCOPY

Carbol-Fuchsin solution according to Kinyoun,for microscopy (Kinyoun's carbol-Fuchsin)

Supplier: Biosensis

Fluoro-Jade stain is a fluorochrome derived from fluorescein, and is commonly used in neuroscience disciplines to label degenerating neurons in ex vivo tissue of the central nervous system.

Supplier: VWR Chemicals

Metyleenisininen 10 g/l in aqueous solution

Supplier: DWK Life Sciences

Soda-lime glass and stainless steel.

Supplier: G-Biosciences

Using the Protein Electrophoresis kit, students will learn the principles of various types of electrophoresis, including denaturing and non-denaturing electrophoresis, and how this powerful technique is used to analyse proteins. The kit will introduce students to the different separation matrices currently in use and will understand their differing separation properties and their role in protein analysis. Students have an option of casting their own electrophoresis gels using polyacrylamide or using pre-cast commercially available gels. This kit is provided with all of the reagents, buffers and supplies needed for casting acrylamide gels, preparing protein samples, running electrophoresis, and staining the gels for visualisation of protein bands. Test protein samples and protein standards are also provided.

Supplier: MP Biomedicals

Benzopurpurin B is used as a biological stain.

Supplier: SIGMA ALDRICH MICROSCOPY

Acid Fuchsin is mainly used as a diachrome and is part of the connective tissue stains. It also works as a pH indicator.

Supplier: SIGMA ALDRICH MICROSCOPY

Bromophenol Blue–Xylene Cyanol Dye solution has been used in the formamide loading buffer for the size purification of ligated RNA from polyacrylamide gel.

Supplier: SIGMA ALDRICH MICROSCOPY

Naphthol AS-BI Phosphoric acid solution foruse in Sigma Acid Phosphatase Procedure No. 386.

Supplier: Biotium

Lumitein™ is a luminescent dye designed for detecting proteins in SDS polyacrylamide (SDS-PAGE) gels. It can also be used to detect proteins in native PAGE gels after an additional SDS incubation step. The stain combines excellent sensitivity, exceptional user-friendliness and compatibility with common instruments and downstream analysis.

Supplier: Biotium

SynaptoGreen™ C18 (equivalent to FM®3-25) is a membrane stain with a chemical structure similar to other nerve terminal dyes, except that it has the longest lipophilic tail (C18). Because of its large size, it is used as a control for ion channel permeability of nerve terminal dyes.