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EasySep™ Red Blood Cell Lysis Buffer

EasySep™ Red Blood Cell Lysis Buffer

Supplier: Stemcell Technologies

EasySep™ Red blood cell lysis buffer is for use as directed with EasySep™ or RoboSep™ whole blood kits for the lysis of red blood cells prior to separation from whole blood.

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Glucose hemolysing solution

Glucose hemolysing solution

Supplier: DIAGNOSTIC SYSTEMS DIASYS

For use with glucose Gluc-DH FS and glucose Gluc-DH FSC.

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I-PER™ Insect Cell Protein Extraction Reagents

I-PER™ Insect Cell Protein Extraction Reagents

Supplier: Thermo Fisher Scientific

The I-PER Insect Cell Protein Extraction Reagent enables gentle and effective extraction of soluble protein from baculovirus-infected insect cells grown in suspension or monolayer culture. The baculovirus insect cell expression system is an efficient and popular system for production of recombinant (eukaryotic) proteins in cell culture. Proteins expressed in baculoviral systems can be used for structural analyses, biochemical assays and a variety of other applications. I-PER Reagent maintains functionality of extracted proteins and is directly compatible with downstream applications such as protein assays, Western blotting and 6xHis-tagged protein purification.

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Lysis buffer, 0,5% Tween 20, pH 8,0, for isolation of His-tag modified proteins

Supplier: Thermo Fisher Scientific

Lysis buffer, 0.5% Tween 20, pH 8.0, for isolation of His-tag modified proteins, Thermo Fisher Scientific

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RBB Buffers

Supplier: OMEGA BIO-TEK

RBB Buffer, 4000 ml, Omega Bio-Tek

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Luciferase cell lysis buffer (2X), Pierce™

Luciferase cell lysis buffer (2X), Pierce™

Supplier: Thermo Fisher Scientific

The Thermo Scientific Pierce luciferase cell lysis buffer effectively lyses adherent cells or pelleted suspension cells for analysis of promoter expression in luciferase assays.

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Protein solubilisation buffer

Protein solubilisation buffer

Supplier: G-Biosciences

FOCUS™ protein solubilisation buffer is a dry, urea-based buffer for protein solubilisation. It is a pre-mixed formulation of urea, thiourea, CHAPS and non detergent sulphobetaine for maximum solubilising strength.

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HEPES lysis buffer with Triton® X-100

Supplier: Thermo Fisher Scientific

HEPES lysis buffer with Triton® X-100, Thermo Fisher Scientific

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Protein extraction reagent, BugBuster® (primary amine-free), Millipore®

Supplier: Merck Millipore (Novagen)

BugBuster® (primary amine-free) Protein Extraction Reagent is a special formulation of BugBuster designed for applications where primary amines would interfere if present in the protein extract, such as protein immobilisation or cross-linking. The PIPPS buffer used in BugBuster® (primary amine-free) has a similar buffer capacity and pH range as the original Tris-buffered BugBuster®, but will not complex metal ions, also making it ideally suited for extraction of metal dependent proteins.

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RBC lysis buffer for mouse

Supplier: Thermo Fisher Scientific

RBC lysis buffer for mouse, Thermo Fisher Scientific

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ReadiUse™ Viral RNA Lysis Buffer

ReadiUse™ Viral RNA Lysis Buffer

Supplier: AAT Bioquest

Current demands for RT-PCR testing of SARS-CoV-2 has led to a worldwide shortage of RNA extraction kits.

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CHAPS lysis buffer

Supplier: Thermo Fisher Scientific

For cell lysis and protein extraction.

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Protein extraction kit, mammalian cell PE LB™

Protein extraction kit, mammalian cell PE LB™

Supplier: G-Biosciences

Mammalian Cell PE LB™ has been developed for extraction of total biologically active, soluble proteins from mammalian cultured cells. The Mammalian Cell PE LB™ is based on organic buffering agents and utilises a mild non-ionic detergent, chelating agent, and a proprietary combination of various salts and agents to enhance extraction and stability of proteins. Depending on the required downstream application, additional agents such as reducing agents, phosphatase and protease inhibitors may be added into Mammalian Cell PE LB™. Mammalian Cell PE LB™ has been tested on a wide variety of mammalian cells and can be used for both suspension and adherent cells.

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Protein extraction kit, bacterial PE LB™

Protein extraction kit, bacterial PE LB™

Supplier: G-Biosciences

Bacterial PE LB™ kit has been developed for the extraction of biologically active soluble proteins, including recombinant proteins and inclusion bodies, from bacterial cells. Bacterial PE LB™ is a proprietary improvement on the lysozyme based lysis method, which allows extraction of soluble proteins and concurrent removal of nucleic acids (DNA and RNA) released during cell lysis. The Bacterial PE LB™ lysis system eliminates viscosity build-up, allowing effective clarification with lower centrifugal forces.

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Denaturing lysis buffer

Supplier: Thermo Fisher Scientific

Denaturing lysis buffer, Thermo Fisher Scientific

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Subcellular Protein Fractionation Kit for Cultured Cells

Subcellular Protein Fractionation Kit for Cultured Cells

Supplier: Thermo Fisher Scientific

This kit is ideal for segregation and enrichment of proteins from five different cellular compartments.

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