Supplier: STEMCELL TECHNOLOGIES

Rat monoclonal antibody against mouse, hamster CD4, APC-conjugated.

Supplier: STEMCELL TECHNOLOGIES

Rat monoclonal antibody against mouse, hamster CD4, unconjugated.

Supplier: STEMCELL TECHNOLOGIES

Mouse monoclonal antibody against human perforin, PE-conjugated.

Supplier: STEMCELL TECHNOLOGIES

Mouse monoclonal antibody against mouse CD45.1, PE-conjugated.

Supplier: STEMCELL TECHNOLOGIES

Rat monoclonal antibody against mouse IL-4, APC-conjugated.

Supplier: STEMCELL TECHNOLOGIES

Count cells with hemocytometer cover glasses designed specifically for use with the Hausser Scientific™ Bright-Line Hemocytometer.

Supplier: STEMCELL TECHNOLOGIES

Rat monoclonal antibody against mouse CD8a, unconjugated.

Supplier: STEMCELL TECHNOLOGIES

Rat monoclonal antibody against mouse CD127, PE-conjugated.

Supplier: STEMCELL TECHNOLOGIES

Rat monoclonal antibody against mouse Ly-6C, PE-conjugated.

Supplier: STEMCELL TECHNOLOGIES

Supplement (50X) for the serum-free culture of neurons.

Supplier: STEMCELL TECHNOLOGIES

Rat monoclonal IgG1, kappa isotype control antibody, PE-conjugated.

Supplier: STEMCELL TECHNOLOGIES

Mouse monoclonal IgG1 antibody against human, rhesus, cynomolgus CD2.

Supplier: STEMCELL TECHNOLOGIES

Mouse monoclonal IgG1 antibody against human, chimpanzee CD3, PE-conjugated.

Supplier: STEMCELL TECHNOLOGIES

Rat monoclonal IgG2b antibody against human, mouse, rhesus CD11b, FITC-conjugated.

Supplier: STEMCELL TECHNOLOGIES

Rat monoclonal IgG2b antibody against human, mouse, rhesus CD11b.

Supplier: STEMCELL TECHNOLOGIES

Rabbit monoclonal IgG antibody against SARS-CoV, SARS-CoV-2 (2019-nCoV) nucleoprotein (HEK293-expressed recombinant).

Supplier: STEMCELL TECHNOLOGIES

Human monoclonal IgG1 antibody against SARS-CoV-2 (2019-nCoV) S protein (HEK293-expressed recombinant).

Supplier: STEMCELL TECHNOLOGIES

96 well high binding flat bottom microplate for radioimmunoassays (RIAs) and enzyme immunoassays (EIAs).

Supplier: STEMCELL TECHNOLOGIES

Rat monoclonal IgG2b, antibody against mouse MHC Class II (I-A/I-E), APC-conjugated.

Supplier: STEMCELL TECHNOLOGIES

Mouse monoclonal IgG1, antibody against human, chimpanzee CD4, unconjugated.

Supplier: STEMCELL TECHNOLOGIES

Immunomagnetic isolation of untouched human memory CD4+ T cells

Supplier: STEMCELL TECHNOLOGIES

Interleukin 3 (IL-3) is a species-specific pleiotropic cytokine that promotes the survival and proliferation of pluripotent hematopoietic stem cells and lineage-committed progenitor cells and their differentiation into mature cells of most lineages, including basophils, neutrophils, eosinophils, macrophages, dendritic cells, erythrocytes, and megakaryocytes (Yang et al.; Dorssers et al.; Broughton et al.). IL-3 is produced by activated T cells and has a physiological role in inflammation and allergies by promoting the secretion of inflammatory mediators such as histamine, IL-4, and IL-6 by basophils and eosinophils (Broughton et al.). The IL-3 receptor consists of a unique alpha subunit (CD123) and a beta common subunit (βc or CD131) that is shared with the receptors for IL-5 and GM-CSF, and is the principal signal transduction subunit for these cytokines. IL-3 binding to the heterodimeric receptor activates JAK/STAT, MAPK, and PI3K signaling pathways (Woodcock et al.).

Supplier: STEMCELL TECHNOLOGIES

Serum-free attachment substrate for the derivation and expansion of human skeletal muscle progenitor cells (myoblasts).

Supplier: STEMCELL TECHNOLOGIES

For culture of human mammary epithelial cells.

Supplier: STEMCELL TECHNOLOGIES

Pre-aliquoted methylcellulose-based medium with recombinant cytokines for human cells. 24×3 ml vials.

Supplier: STEMCELL TECHNOLOGIES

Mediate calcium phosphate clearance and prevent ectopic calcification with fetuin A, a plasma glycoprotein that forms soluble complexes with calcium and phosphate (Heiss et al.; Price and Lin). Belonging to the cystatin superfamily of cysteine protease inhibitors (Brown and Dziegielewska), fetuin A has also been shown to play a role in lipid transport, acting as a carrier (Kumbla et al.). In cell-based assays, it has been suggested that fetuin A protects against lethal systemic infection through the inhibition of high mobility group box 1 (HMGB1) protein accumulation and release (Li et al.). Fetuin A acts as a natural antagonist against specific TGF-β and BMP signaling proteins, blocking osteogenic differentiation of rat bone marrow cells (Demetriou et al.). This protein contains a His-residue tag at the carboxyl end of the polypeptide chain. For consistency and reproducibility across your applications, fetuin A from STEMCELL comes lyophilised with ≥94% purity, and endotoxin levels are verified to be ≤1.0 EU/μg protein.

Supplier: STEMCELL TECHNOLOGIES

Flt3/Flk-2 (Fms-like tyrosine kinase 3/fetal liver kinase-2) ligand is a hematopoietic cytokine that plays an important role as a co-stimulatory factor in the proliferation, differentiation, and survival of hematopoietic stem and progenitor cells and the development of the immune system (Lyman et al.; Rosnet et al.). Flt3/Flk-2 ligand, together with stem cell factor and thrombopoietin, is commonly used to promote expansion of primitive hematopoietic cells in culture. In combination with myeloid cytokines such as GM-CSF, G-CSF, or M-CSF, Flt3/Flk-2 ligand enhances the growth and numbers of clonogenic myeloid progenitor cells. In synergy with IL-3, IL-4, IL-7, IL-11, IL-12, IL-15, GM-CSF, and TNF-α, Flt3/Flk-2 ligand regulates the development of various lymphoid progenitor cells, including dendritic cell, B cell, T cell, and NK cell progenitors. In contrast, Flt3/Flk-2 ligand has no significant effect on erythropoiesis or megakaryopoiesis (Drexler and Quentmeier; Wodnar-Filipowicz). Flt3/Flk-2 ligand exists as membrane-bound and soluble isoforms. Both isoforms are biologically active and signal through the class III tyrosine kinase receptor (Flt3/Flk-2, CD135; Rosnet et al.). Flt3/Flk-2 ligand is produced by a variety of cell types, including uncommitted and committed hematopoietic cells and stromal fibroblasts, whereas expression of the Flt3/Flk-2 receptor is restricted to CD34+ hematopoietic stem and progenitor cells. Flt3/Flk-2 receptor is also expressed outside the hematopoietic system in the brain, placenta, and testis (Drexler and Quentmeier; Hannum et al.).

Supplier: STEMCELL TECHNOLOGIES

Interleukin 15 (IL-15) is a four-alpha helix bundle cytokine with many similar properties to IL-2, with which it shares components of its receptor. The IL-15 receptor is a heterotrimeric receptor composed of IL-15Ra, the high-affinity receptor for IL-15, as well as IL-2/15Rb (CD122) and common gamma chain (CD132). IL-15 binds to IL-15Rα receptor and can then be presented in trans to IL-2/15Rb and common gamma chain on other cells. Trans-presentation is thought to be the major mechanism by which IL-15-mediated responses occur in mice, although may not be necessary in humans (Castillo et al.). The cytoplasmic domains of IL-2/15Rb and common gamma chain mediate signaling to activate JAK/STAT and PI3K pathways. IL-15 supports the survival and proliferation of naive CD4+ and CD8+ T cells, and promotes homeostasis of memory T cells. IL-15 also promotes the survival and differentiation of NK cells and regulates their cytolytic activity (Ma et al.).

Supplier: STEMCELL TECHNOLOGIES

Rat monoclonal IgM antibody against mouse CD49b (integrin α2), PE-conjugated.

Supplier: STEMCELL TECHNOLOGIES

Density gradient medium for use with RosetteSep™ CD3+ T cell, CD4+ T cell, CD8+ T cell, and B cell enrichment cocktails.