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1066 Results for: "SIGMA ALDRICH MICROSCOPY"

Sulpho-SMCC (3-Sulpho-N-succinimidyl-4-(N-maleimidomethyl)cyclohexane-1-carboxylate sodium salt), Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

4-(N-Maleimidomethyl)cyclohexane-1-carboxylic acid 3-sulfo-N-hydroxysuccinimide ester sodium salt,powder (Sulfo-SMCC)

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Tissue freezing medium, PolyFreeze, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Tissue samples may be snap frozen using PolyFreeze with isopentane and liquid nitrogen, dry ice (slush/slurry or bunker), or in a cryostat. PolyFreeze media do not cause autofluorescence and can easily be washed away during tissue fixation when rinsed prior to staining. Once rinsed off the slide, there is no trace of the support matrix to interfere with staining or immunohistochemistry (IHC) reactions.

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Hydroquinone

Hydroquinone

Supplier: SIGMA ALDRICH MICROSCOPY

Intended for use in the Silver Stain (Steiner-Steiner) kit. Hydroquinone, 0.5 g each, with excipients.

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Organo/Limonene Mount™

Organo/Limonene Mount™

Supplier: SIGMA ALDRICH MICROSCOPY

Organo/Limonene Mount™ is a ready-to-use mounting medium is made with limonene, a natural product from orange peels. It is good for coverslipping stained tissues and cell smears that can be dehydrated with organic solvents.

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Crystal violet 1% in aqueous solution, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Crystal violet solution for use in Brown-Hopps method for Gram-positive and Gram-negative bacterial staining.
Crystal violet solution has been used as a stain in cell proliferation assays, migration assays and Boyden chamber assay.

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Tuerk solution for microscopy, for leukocyte count, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Counting leukocytes in the counting chamber.

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Morin hydrate 1-2 mol/mol water for the determination of Al, Be, Zn, Ga, In, Sc, for microscopy, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Morin hydrate is a yellowish pigment and a fluorescent indicator. It is used for the fluorimetric determination of several metals, mostly aluminum. Morin has been utilised in fluorescent microscopy as a fluorescent nuclear stain and as an application to detect aluminium in the brain by demonstrating metal salts.

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Gram's decolourising solution for Gram staining, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Gram staining is one of the most significant staining techniques in microbiology. Gram′s decolouriser solution is a mixture of ethanol and acetone. Gram′s decolouriser solution is used in Gram staining procedure, after the iodine step to remove the crystal violet dye. The decolouriser dissolves the lipid layer from the gram-negative cells.

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Schaeffer and Fulton Spore Stain Solution B, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

In Schaeffer-Fulton`s method, a primary stain-malachite green is forced into the spore by steaming the bacterial emulsion. Malachite green (Schaeffer & Fulton`s Spore Stain A: Cat. No. 90903) is water soluble and has a low affinity for cellular material, so vegetative cells may be decolorized with water. Vegetative cells are then counterstained with safarin. The components and the method are also available as kit (Cat. No. 04551).
Schaeffer & Fulton`s Spore Stain B: SafarinO 5 g/l in water

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Aqueous mounting medium, Fluoroshield™ with 1,4-Diazabicyclo[2.2.2]octane, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Fluoroshield™ with 1,4-Diazabicyclo[2.2.2]octane is an aqueous mounting medium for preserving fluorescence of tissue and cell smears.

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Bromobimane (mBBr) ≥97%, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Bromobimane is also known as monobromobimane. It is also a known probe for thiols and is a fluorescent reagent activated upon a photolysis reaction.

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Naphthol AS-BI Alkaline solution, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Kit component for Sigma Alkaline Phosphatase Procedure, kits 86C and 86R

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Eriochrome Black T, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Eriochrome Black T, Sigma-Aldrich®

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Acid alcohol solution, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

This acid alcohol is part of Flourecent Stain Kit for Mycobacteria (Cat. No. 05151). It is used for decolorising.

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Azure B (azure I) ≥85% (by HPLC), prepared by direct synthesis, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Azure B has been used in M′Fadyean stain to visualize Bacillus anthracis capsule. Azure B is a cationic dye. It is part of the Romanowsky stain, which is used for staining of blood films. It is also used for the localisation of nucleic acids and lignin.

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Gram′s fuchsin solution for microscopy, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

May contain minor quantities of precipitated dye.

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PAS (Periodic Acid Schiff) staining kit, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Standard procedure and microwave procedure included in package insert.
When treated with periodic acid, glycols are oxidized to aldehydes. After reaction with Schiff′s Reagent (a mixture of pararosaniline and sodium metabisulfite), a pararosaniline adduct

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α-Naphthyl Butyrate Esterase, Lymphocyte kit, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

This kit is intended for demonstration of nonspecific esterase in a subset of lymphocytes for determining maturation arrest. Kits 90A1 or 91A are intended for monocyte identification.

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Metanil yellow 70% (dye content), Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Metanil yellow has been used for staining of leukocyte granules. It is also used as a counterstain in microscopic sections.

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Bromocresol green sodium salt 0.04% in aqueous solution, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Bromocresol green is a sulfonephthalein dye. It is mainly used for measuring serum albumin. In acidic conditions, bromocresol green interacts with albumin, thereby resulting in an increase in absorbance at 630 nm. The change in absorbance is linked with the concentration of albumin. Bromocresol Green is also a pH indicator, changing from yellow (pH 3.8) to blue color (5.4). It is widely used as a colloidal pigment.

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Pinacyanol chloride, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Pinacyanol chloride, (1,1'-Diethyl-2,2'-carbocyanine chloride; 2,2'-Trimethinequinocyanine chloride; Quinaldine blue).

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Peroxidase Indicator Reagent

Supplier: SIGMA ALDRICH MICROSCOPY

It contains p-Phenylenediamine diHCL (1 part) and catechol (2 parts). Commonly referred to as Hanker Yates reagent. Kit component in the Sigma Leukocyte peroxidase (myeloperoxidase) Procedure, 390A.

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Peroxidase (Myeloperoxidase) Leukocyte Kit Hanker-Yates substrate kit, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Staining system for polymorphonucleocytes in blood or bone marrow films.

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Histopaque®, sterile filtered, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

ACCUSPIN System-Histopaque-1077 products are intended for use in the isolation of lymphocytes and other mononuclear cells. The separation medium, Histopaque-1077, is a sterile-filtered, endotoxin-tested solution of polysucrose and sodium diatrizoate, adjusted to a density of 1,077 g/ml. ACCUSPIN System-HISTOPAQUE-1077 employs γ-irradiated 12mL polypropylene centrifuge tubes specially designed with two chambers separated by a porous high-density polyethylene barrier ('frit'). Each tube is aseptically filled with 3 mL of Histopaque 1077. Each tube will separate 3 to 6 ml of anticoagulated blood.

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4-Amino-3-hydroxy-1-naphthalenesulphonic acid in ACS Reagent grade water ≥90%, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

4-Amino-3-hydroxy-1-naphthalenesulfonic acid is a derivative of aniline consisting of a trifunctional monomer (bearing three functional groups –NH₂, –OH and –SO₃H). It also contains two fused benzene rings.

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Indophenol blue 60% (dye content), Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Indophenol Blue is used to colourimetrically measure N-containing compounds. The free amino acids react to form a blue color complex.

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Haematoxylin solution (Mayer), Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

General purpose nuclear stain, progressive type. Used with hematoxylin and eosin staining.

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Poly-L-lysine hydrobromide

Supplier: SIGMA ALDRICH MICROSCOPY

Poly-L-lysine is a positively charged amino acid polymer with approximately one HBr per lysine residue. The hydrobromide allows the poly-L-lysine to be in a crystalline form soluble in water. A small amount of product may be found in the beta structure because the HBr interferes with hydrogen bonding between amino and either the carboxyl groups or N or O containing moieties.

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Chromazurol B

Supplier: SIGMA ALDRICH MICROSCOPY

Chromeazurol B is a dye, belonging to the class of triphenylmethanes.

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Pararosaniline, crystalline, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Pararosaniline Base,crystalline (Parafuchsin; Paramagenta)

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