342 tulosta haulle: "EDVOTEK"

Supplier: EDVOTEK

Four experimental modules are combined into one experiment to provide a comprehensive biotechnology exploration focusing on the green fluorescent protein (GFP). Bacterial cells are transformed to express the GFP. The transformed cells are then grown and the GFP purified by column chromatography. Finally, the purity of the protein fractions are analysed by SDS polyacrylamide electrophoresis.

Supplier: EDVOTEK

When bacteria are used to make medicinally useful proteins by transformation, the protein of interest must be separated from all of the other cellular proteins. In this experiment, the unique fluorescent properties of GFP and BFP are used as an assay during their purification from an E. coli extract. The column fractions containing GFP or BFP are identified by fluorescence and then purified. As an optional activity, purified protein fractions can be separated by SDS polyacrylamide gel electrophoresis (SDS-PAGE) to estimate the purity and size of the GFP and BFP proteins.

Supplier: EDVOTEK

The Green Fluorescent Protein (GFP) from the jellyfish Aequorea victoria is used extensively in all areas of science. Many organisms have been transformed with the GFP gene, the gene responsible for bioluminescence in jellyfish. It has proven to be so useful that scientists have mutated it to produce Blue Fluorescent Protein (BFP). In this simple experiment, students transform bacteria either by GFP, BFP or both.

Supplier: EDVOTEK

Capable of running one or two groups of student samples at the same time and producing excellent results in 30 to 40 minutes.

Supplier: EDVOTEK

Water baths incorporating digital temperature control and an optional shaking capacity (10 L model only).

Supplier: EDVOTEK

This lightweight, cordless pipetting controller is ideally suited as an aliquoting tool for instructors and teaching assistants. It uses all standard serological pipettes. The speed can be fine-tuned by applying varying finger pressure to the operating buttons.

Supplier: EDVOTEK

This experiment uses PCR to amplify a small section of Lambda DNA via a 2-step process, saving valuable classroom time and allowing completion of the lab in one session.

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Manual pipette tip, Filtered: -, Non sterile, 1 - 200 µl, Fine tip, Transparent, 2 racks, 96 each

Supplier: EDVOTEK

Sturdily designed handheld pipettes with volumes ranging from 0,1 to 5000 µl. They are easy to use, accurate and use standard micropipette tips.

Supplier: EDVOTEK

Experimentation kits, human PCR Tool Box™

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This simulation experiment demonstrates the process of DNA amplification by PCR and how the amplified product is detected by separating the reaction mixture by agarose gel electrophoresis.

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Ready-to-Load™ kit to show how electrophoresis separates molecules on the basis of size and charge.

Supplier: EDVOTEK

Link important STEM concepts using Agarose Gel Electrophoresis.

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Digital stainless steel shaking water bath, designed for when incubation of samples together with precise aeration and agitation is required.

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The objective of this experiment is to investigate the genetic basis of beak size variation in Galapagos finches before and after a drought event using Restriction Fragment Length Polymorphism (RFLP) analysis.

Supplier: EDVOTEK

Hands-on simulation of CRISPR-Cas9 to target and treat Cystic Fibrosis.

Supplier: EDVOTEK

This LabStation provides all that is needed to run any Edvotek DNA or dye electrophoresis kits with students.

Supplier: EDVOTEK

In this experiment, students study the diversity of fish using pre-stained, lyophilised proteins. Total protein from perch, walleye and salmon is extracted and pre-stained using an indicator dye. Each fish protein sample has a characteristic banding pattern when separated by denaturing SDS-polyacrylamide gel electrophoresis, which can be used to identify the specific species.

Supplier: EDVOTEK

Coronary heart disease and stroke are major causes of death in the Western world. Elevated blood cholesterol levels are a serious risk factor in both conditions. The genetic disease familial hypersholesterolemia (FH) causes an increase in blood levels of the bad form of cholesterol, low density lipoprotein (LDL). In untreated patients with the mutant FH gene, the condition can cause premature death. This experiment introduces the colorimetric enzymatic reaction which is the basis of the clinical cholesterol test. In addition, using agarose gel electrophoresis, students can analyse a simulated genetic screening for a disease.

Supplier: EDVOTEK

Most molecules have a net charge within a pH range of 2 to 10. When the pH is altered, the net charge on molecules can change drastically. In this experiment, a mixture of two chemicals is absorbed onto a solid support ion-exchange column and separated during elution under conditions that influence their net charge.

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Students learn the basic principles of the Enzyme-linked Immunosorbent Assay (ELISA) using this precise and sensitive antibody-based detection kit.

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This experiment introduces chromatographic theory and methods of thin layer chromatography (TLC). A mixture of dyes are separated on a cellulose-based TLC plate using different solvent systems.

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Controller, pipette, Blue

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Pipette, Pasteur / transfer

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Students learn about DNA polymorphisms by amplifying DNA regions that vary between wild and mutant Drosophila. Amplified DNA from wild-type and white-eyed flies are separated by agarose gel electrophoresis and analysed.

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EVT 300 power supplies can operate M6Plus units, two M12 units or two HexaGels (at 75 V). The EVT 300 runs gels as fast as 20 to30 minutes (at 150 V).

Supplier: EDVOTEK

This kit is for teaching students how people's physical traits are a reflection of their genes. In this simulation, students use electrophoresis to separate dyes which represent genetic traits.

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Six groups of students can load their own individual gels and the six gels can be run together in 30 to 40 minutes. No need for gel taping and gels can be poured quickly and easily using the gel tray sealing rubber end caps.

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Scents and sense-ability kit

Supplier: EDVOTEK

Investigate how agarose gel electrophoresis unlocks the color code used by food scientists to make colorful candies.