"Cytiva"
GST buffer kit
Supplier: Cytiva
GST Buffer Kit contains carefully prepared buffer concentrates for binding, washing, and elution of GST-tagged proteins according to recommended protocols.
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Magnetic Beads, Sera-Mag SpeedBeads Streptavidin-blocked magnetic particles
Supplier: Cytiva
Sera-Mag SpeedBeads Streptavidin-Blocked Magnetic Particles combine high affinity for biotinylated target molecules with very low non specific binding. Sera-Mag SpeedBeads Streptavidin-Blocked Magnetic Particles combine fast reaction kinetics with very low non specific binding for increased throughput and precision. Applications include automated immunoassays, immunoprecipitation, and protein purification. Compounds that are difficult to attach to particle surfaces by conventional means may be amenable to biotinylation. Sera-Mag SpeedBeads and Sera-Mag Streptavidin-Coated Magnetic Particles provide a high biotin-binding capacity along with a strong affinity for targeted, biotin labeled molecules.
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DNA (Deoxyribonucleic acid) (from salmon testes), sonicated
Supplier: Cytiva
Genomic DNA sheared by sonication for use in hybridization experiments
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Monofunctional maleimide fluors, CyDye™
Supplier: Cytiva
Cy3 and Cy5 monofunctional maleimides are particularly suitable for the selective labelling of molecules containing free sulfhydryl groups, such as cysteine residues in proteins and peptides and oligonucleotides.
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illustra™ plasmidPrep Mini Spin kit
Supplier: Cytiva
The illustra™ plasmidPrep Mini Spin Kit uses a simple plasmid DNA purification protocol involving a modified alkaline lysis procedure and a novel silica-based membrane to achieve highly efficient plasmid DNA purification. Produces high quality plasmid DNA with excellent reproducibility for use in cloning, restriction enzyme digestion, PCR amplification, and DNA sequencing.
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Anti-IgG Goat Antibody (CyDye™ 700)
Supplier: Cytiva
Amersham CyDye NIR-labeled secondary antibodies enable quantitation of Western blots with a strong signal-to-noise ratio. Mulitplexed experiments allow for the detection of two proteins on a single blot. The use of total lane signals to normalize target protein data yield robust and reliable results.
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Antibody column package, Modern Protein A (2-step purification protocol)
Supplier: Cytiva
The Antibody column package includes modern protein A affinity and size exclusion chromatography columns to obtain highly pure human IgG.
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Amersham CyDye DIGE fluors
Supplier: Cytiva
CyDye™ DIGE fluors are available as minimal and saturation labeling dyes. The minimal dyes are intended for general 2-D application use where sufficient amounts of sample are available. The saturation dyes from the Scarce Sample Labeling Kit are designed to be used for applications where only small amounts of sample are available, for example in Laser Capture Microdissection.
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HiTrap™ Capto™ Octyl Hydrophobic Interaction Chromatography Column
Supplier: Cytiva
Capto™ Octyl are hydrophobic interaction chromatography (HIC) resins used in the capture and intermediate stages of protein purification.
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Western blotting markers, Amersham™ ECL™ DualVue™
Supplier: Cytiva
Amersham™ ECL™ DualVue™ Western blotting markers perform two key functions. Firstly, the prestained marker proteins confirm protein transfer in addition to clearly defining blot orientation. Secondly, the recombinant tagged proteins are detected on film or by CCD imaging in conjunction with the target protein(s) of interest, enabling highly accurate molecular mass determination of the target protein(s). The tagged markers are detected easily and specifically by means of a specific conjugate that eliminates any cross contamination between target protein(s) and markers.
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Affinity chromatography columns, HiScreen™ Ni FF
Supplier: Cytiva
HiScreen columns prepacked with Ni Sepharose 6 Fast Flow for method optimization and parameter screening for purification of histidine-tagged proteins with Immobilized Metal ion Affinity Chromatography (IMAC).
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Streptavidin, horseradish peroxidase complex, biotinylated
Supplier: Cytiva
Streptavidin, horseradish peroxidase complex, biotinylated
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CyDye™ Mono-Reactive Maleimide Fluorescent Dyes, Cytiva
Supplier: Cytiva
CyDye™ Mono-Reactive Maleimide Fluorescent Dyes are intensely fluorescent and highly water soluble, providing significant advantages over other existing fluorophores.
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Gel filtration chromatography columns, HiScale™ Superdex™ Increase and Superose™ Increase
Supplier: Cytiva
These HiScale columns packed with Superdex Increase or Superose Increase resins are used to purify larger volumes of up to 8.5 ml.
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Isoelectric focusing system, Ettan™ IPGphor™ 3
Supplier: Cytiva
Ettan™ IPGphor™ 3 is a fully integrated isoelectric focusing (IEF) system optimised to deliver high throughput, speed, reproducibility, and high protein-loading capacity. Ettan™ IPGphor™ 3 is optimised for easy handling of CyDye™ labelled proteins and other light-sensitive samples, and provides temperature control critical for reproducibility in 2-D DIGE experiments.
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Hydrophobic interaction chromatography column, HiTrap™ Capto™ Phenyl ImpRes
Supplier: Cytiva
HiTrap Capto Phenyl ImpRes is prepacked with Capto Phenyl ImpRes media for screening and small-scale protein purifications using hydrophobic interaction chromatography.
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