"Alsto"
VWR®, Trays for VLSB Shaking Baths
Supplier: VWR Collection
Shaking bath, Test tube tray, (also used as plain tray) stainless steel for 3 test tube racks, for VLSB12
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Reactive oxygen species indicators dyes
Supplier: Biotium
Dihydrorhodamine 123 is the reduced form of rhodamine 123, which is a commonly used fluorescent mitochondrial dye. It is non-fluorescent, but it readily enters most of the cells and is oxidized by oxidative species or by cellular redox systems to the fluorescent rhodamine 123 that accumulates in mitochondrial membranes. It is useful for detecting reactive oxygen species including superoxide (in the presence of peroxidase or cytochrome c) and peroxynitrite. Dihydrorhodamine 123 dihydrochloride is functionally equivalent to dihydrorhodamine 123 but with increased stability toward air oxidation and light during storage. Dihydroethidium (also called hydroethidium) is the chemically reduced form of the commonly used DNA dye ethidium bromide. The probe is useful to detect oxidative activities in viable cells, including respiratory burst in phagocytes. Dihydroethidium itself has blue fluorescence (355/420 nm) in cells, while the oxidized form ethidium has red fluorescence (518/605 nm) upon DNA intercalation. H2DCFDA (2′,7′-Dichlorodihydrofluorescein diacetate) is a useful fluorogenic reagent to detect reactive oxygen intermediates in cells. On oxidation, H2DCFDA becomes the highly green fluorescent 2′,7′-dichlorofluorescein, 504/529 nm (end product).
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1,4-Dithioerythritol ≥99%, white crystalline powder
Supplier: MP Biomedicals
Dithioerythritol is an erythro isomer of 2,3-dihydroxy-1,4-dithiolbutane, and an isomer of 1,4-dithiothreitol which is a reagent for maintaining thiols in the reduced state; prevents oxidation of sulfhydryl-containing proteins during SDS-polyacrylamide gel electrophoresis. It also quantitatively reduces disulfides.
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Furaptra (Mag-Fura-2) AM Ester ≥95% (by TLC) fluorescent indicator, for calcium determination
Supplier: Biotium
Furaptra, AM ester is a membrane-permeant form of Furaptra (Mag-Fura-2). The indicator is loaded into cells via simple incubation
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Amplite® Fluorimetric Neuraminidase Assay Kit *Blue Fluorescence*
Supplier: AAT Bioquest
Neuraminidases, also called sialidases, are glycoside hydrolase enzymes that catalyze the hydrolysis of terminal sialic acid residues and neuraminic acid.
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Human recombinant ENA 78/CXCL5 (from E. coli)
Supplier: Shenandoah Biotechnology
Epithelial-derived neutrophil-activating peptide 78 (ENA 78), also known as CXCL5, is a chemokine that recruits neutrophils, promotes angiogenesis.
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Human Recombinant MIP-3 B (from E. coli)
Supplier: Shenandoah Biotechnology
Macrophage inflammatory protein-3 beta (MIP-3 β), also called CCL19, is a chemokine that is expressed in the thymus, lymph nodes, and activated bone marrow stromal cells. MIP-3 β signals through the G protein-coupled receptor CCR7 to regulate normal lymphocyte recirculation. MIP-3 β also functions during T cell trafficking to the thymus, and in T cell and B cell homing to the lymph nodes and secondary lymphoid organs. Human MIP-3 β shows activity on mouse cells.
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Triethylammonium bicarbonate buffer, for HPLC, LiChropur™, Supelco®
Supplier: Merck
1M Buffer, also for use in ion-exchange chromatography and electrophoresis.
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Amplite® Colorimetric Enterokinase Activity Assay Kit
Supplier: AAT Bioquest
Enterokinase (also called enteropeptidase) is a serine protease produced by cells in the duodenal wall and is a key enzyme in human and animal digestion system.
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Amplite® Fluorimetric Acetylcholinesterase Assay Kit *Red Fluorescence*
Supplier: AAT Bioquest
Acetylcholinesterase, also known as AChE, is an enzyme that degrades (through its hydrolytic activity) the neurotransmitter acetylcholine, producing choline and an acetate group.
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4-Nitrophenyl acetate, pale yellow powder
Supplier: MP Biomedicals
p-Nitrophenyl Acetate used with iodoacetic acid for reductive cleavage of methionine-containing peptides. It is also used as a substrate that has been used in assays for esterase and lipase activity. Inorganic complexes have been evaluated for their methanolysis or hydrolysis activity using p-nitrophenyl acetate.
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Organic reference standard, epsilon-Caprolactam, analytical standard, Supelco®
Supplier: Merck
ε-Caprolactam is used in the manufacture of polyamides. It is also used to produce nylon 6.
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Acriflavine fluorescent labeling reagent, Sigma-Aldrich®
Supplier: SIGMA ALDRICH MICROSCOPY
Acriflavine also interacts with HIF-1α (hypoxia-inducible factor 1 α) and HIF-2α, and thereby suppresses dimer formation of HIF-1 and transcriptional function. It also has negative effect on tumor growth and vascularization. Acriflavine also exhibits trypanocidal, antibacterial and antiviral activities.
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Glutaraldehyde 25% in aqueous solution
Supplier: MP Biomedicals
Glutaraldehyde is also referred to as glutaral, 1,5-pentanedione, potentiated acid glutaraldehyde, sonacide, and glutardialdehyde. Pure monomeric glutaraldehyde has an absorbance peak at 280 nm and the main impurity, possibly a polymer, has an absorbance peak at 235 nm. Upon analysis, this product is 25-28% by titration and has an A235/A280 ratio of not more than 0,5. Monomeric glutaraldehyde may be purified from polymeric glutaraldehyde by treatment with charcoal (5% w/v) and subsequent filtration (3-4 times). Untreated glutaraldehyde has an absorption at 235 nm that is 5 times greater than that at 280 nm, whereas, after three washings the values are about equal. Glutaraldehyde is a bifunctional cross-linking reagent, reacting with NH2 groups to form Schiff's bases.
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RH 237 [N-(4-Sulphobutyl)-4-(6-(4-(dibutylamino)phenyl)hexatrienyl)pyridinium inner salt]
Supplier: AAT Bioquest
RH 237, also called N-(4-sulfobutyl)-4-(6-(4-(dibutylamino)phenyl)hexatrienyl)pyridinium, is a neuron-tracing dye.
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Human Recombinant MCP-2 (from E. coli)
Supplier: Shenandoah Biotechnology
Monocyte chemotactic protein 2 (MCP-2), also known as CCL8, is a cytokine that is important during allergic and inflammatory responses. MCP-2 activates mast cells, eosinophils, and basophils through the G protein-coupled chemokine receptors CCR1, CCR2B, and CCR5. MCP-2 signaling through the CCR5 receptor also functions as a natural inhibitor of the human immunodeficiency virus, type 1 (HIV-1).
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