Affinity chromatography columns, HiTrap™ MabSelect™ PrismA
Product Details & Documents
These columns are prepacked with MabSelect™ PrismA protein A chromatographyn resin that has been improved with an optimised high-flow agarose base matrix and a genetically engineered protein A-derived ligand, allowing efficient cleaning between purification runs. These columns are suitable for purifying antibodies with affinity chromatography in research and process development.
- Enhanced binding capacity compared with other Protein A resins
- Excellent alkaline stability enables efficient cleaning and sanitisation using 0,5 to 1,0 M NaOH
- Convenient HiTrap™ format for easy connection to a syringe, peristaltic pump, or chromatography system such as an ÄKTA system
MabSelect™ PrismA builds on the proven track record of MabSelect™ and MabSelect™ SuRe™ resins in commercial mAb production.
The columns are made of biocompatible PP that does not interact with biomolecules.
: Note that HiTrap™ columns cannot be opened or repacked.
Specifications
- Flow rate:<20 ml/min
- Particle Size:60 µm
- Capacity:5 ml
- Description:HiTrap™ MabSelect™ PrismA
- Seq:04
- Column hardware:PP
- Bed dimensions:16×25 mm
- Cat. No.:17-5498-54
- Max. pressure:5 bar
- Binding capacity:~ 40 mg polyclonal IgG/ml resin, 2 min residence time
~ 80 mg polyclonal IgG/ml resin, 6 min residence time - Storage:20% ethanol, 2 to 8 °C
- Temperature limit:40 °C
- pH range:3 – 12
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