CleanScribe™ RNA Polymerase is a novel enzyme that reduces double-stranded RNA (dsRNA) formation in IVT RNA synthesis.

• Lowers double-strand RNA (dsRNA) formation by up to 85% during in vitro transcription (IVT)

  Provides simple IVT integration by directly substituting wild-type T7 RNA polymerase in protocols

  Requires little to no optimisation of IVT protocols, unlike other available mutant enzymes

  Grade: Molecular biology

TriLink’s CleanScribe™ RNA Polymerase is a novel DNA-dependent RNA polymerase that catalyses the in vitro transcription (IVT) of a recombinant gene regulated by the T7 promoter. This enzyme reduces double-strand RNA (dsRNA) levels by up to 85% during IVT. dsRNA is a byproduct of IVT reactions and can trigger undesirable inflammatory responses in host cells.

This novel RNA polymerase can directly substitute a wild-type T7 RNA polymerase in an IVT protocol to synthesize messenger RNAs (mRNAs) from a DNA template. It is highly efficient and specific, capable of transcribing large quantities of RNA in a relatively short time frame. Its robustness, reproducibility, and dsRNA reduction make it ideal for mRNA synthesis as well as other applications such as saRNA synthesis, radiolabeled RNA probe preparation, and RNA construct development for additional studies.

The 100 and 840 µg pack sizes contain approximately 50000 units and 420000 units of enzymes, respectively. One unit of enzyme incorporates 1 nmol of ATP in 1 hour at 37 °C.