Pellet Paint® NF Co-Precipitant is a non-fluorescent dye-labelled carrier compatible with fluorescent sequencing. It facilitates rapid removal of BigDye® Terminators during the alcohol precipitation of cycle sequencing reaction products. Cycle sequencing reactions can be precipitated rapidly with centrifugation times of 10 minutes. The easily visualised carrier provides a simple confirmation that precipitation has occurred. Sequencing reaction products are efficiently pelleted and dye-labelled terminators remain in the supernatant during alcohol precipitation using the standard Applied Biosystems precipitation protocols. Re-suspension of pelleted sequencing reaction products in deionised formamide can be confirmed by checking for dissolution of the carrier pellet.

• Rapid and efficient non-fluorescent visible DNA co-precipitant for automated sequencing applications
• Efficient removal of dye terminators
• Direct visualisation and tracking of precipitated material
• No effect on sequencing reaction or sequence accuracy
• Suitable as a substitute for Pellet Paint® co-precipitant for fluorescent detection applications

To avoid extra sample handling, Pellet Paint® NF co-precipitant can be added directly to the reaction mix, template DNA, crude PCR samples, or dilution buffer prior to the cycle sequencing reaction.