From ALiCE® for Research to ALiCE® for Scale-Up Protein Expression, we offer cell-free protein expression products with radical results for every production setup.
- Produces a minimum of 2 mg/ml protein in under 48 hours
- Simple reaction set-up - just add DNA
- Capable of catalysing complex post-translational modifications
The ALiCE® kits were developed specifically for ease of use. Expression of your protein of interest follows a simple four-step procedure:
1. Clone your gene of interest into the ALiCE® vector of your choosing
2. Add your plasmid DNA to a thawed vial of ALiCE® lysate
3. Incubate at 25 °C with aeration for 24 - 48 h
4. Collect your protein
For the full details of the procedure, download our user manual.
ALiCE® for Research-Mini kit contains reagents for 6×50 µl ALiCE protein expression reactions:
6×50 µl ALiCE lysate aliquots
pALiCE01 vector
pALiCE02 vector
6×ALiCE reaction tubes
Short protocol
ALiCE® for Research-Midi kit contains regents for protein cell free expression reactions:
6×200 µl ALiCE lysate aliquots
pALiCE01 vector
pALiCE02 vector
24×ALiCE reaction tubes
Short protocol
ALiCE® for Research-Mixi kit contains reagents for 60×500 µl ALiCE protein expression reactions:
6×500 µl ALiCE lysate aliquots
pALiCE01 vector
pALiCE02 vector
60×ALiCE reaction tubes
Short protocol
Storage Conditions: They should be transferred directly to a –80 °C freezer upon delivery.