Brain Lysate (7 Days Old)
Product Details & Documents
Brain tissue lysate (7 Days Old) was prepared by homogenisation in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7,4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0,1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin.
Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6,8, 12,5% glycerol, 1% sodium dodecylsulfate, 0,01% bromophenol blue) containing 5% β-mercaptoethanol.
Specifications
Specifications
- Cell Format:Cryopreserved
- Pk:0,1 mg
- Description:Normal; Source: Mouse; Tissue: Brain
- Cat. No.:PRSI1403-7
- Storage conditions:Store at 2...8 °C for continuous use. For extended storage, freeze working aliquots at −70 °C.
- Concentration:2 mg/ml
- Tested applications:WB
- Storage buffer:Brain lysate (7 Days Old) is supplied in SDS sample buffer containing 5% β-mercaptoethanol
Specifications
#: 20864.320
Supplier: VWR Chemicals
#: 83640.320
Supplier: VWR Chemicals
#: 1.00030.2500
Supplier: Merck
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Supplier: VWR Chemicals
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Supplier: VWR Chemicals
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Supplier: Merck