DNA ladders in TE buffer are used for sizing linear double-stranded DNA fragments separated by gel electrophoresis. The 1 kb DNA ladder is suitable for sizing DNA fragments from 250 bp to 10 kb, while the 100 bp DNA ladder is suitable for sizing DNA fragments from 100 bp to 1500 bp.

The 1 kb DNA ladder is suitable for sizing linear double-stranded DNA fragments from 250 bp to 10 kb. The 1 kb and 3 kb bands have increased intensity to provide internal orientation. When 100 ng of 1 kb ladder is loaded, the reference bands will contain ~16 ng of DNA per band, while the other bands will contain ~6 ng of DNA per band.

The 100 bp DNA ladder is suitable for sizing linear double-stranded DNA fragments from 100 bp to 1500 bp. The 500 bp band has increased intensity to provide internal orientation. When 100 ng of 100 bp ladder is loaded, the reference band will contain ~23 ng of DNA, while the other bands will contain ~7.7 ng of DNA per band.