SUPREME Real time detection uses real-time PCR for the detection of DNA present in the mitochondrial genome in a simple, reliable, and rapid procedure. The assay is based on 5’ nuclease real time PCR reactions to amplify a unique genomic sequence in the target.

• Amplification and detection of DNA
• Real-time PCR with rapid hot-start Taq DNA polymerase
• Internal Control to exclude false-negative results
• Optimised to handle PCR inhibitors, containing UDG enzyme
• Higher stability, enzymes in seperate tubes
• PCR- platforms: runs on all established standard real-time PCR- platforms
• Harmonised thermal profiles to run with other SUPREME BIOPREMIER kits simultaneously
• Channel Target: FAM, Channel IC: ROX
• Available formats: Kits with 100 or 10 reactions

Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the target as well as a positive control and an internal control (IC). To minimise PCR cross-contamination, the Kit contains dUTP and uracil-N glycosylase (UDG). Comparative quantification analysis of DNA in food and feedstuff products to estimate the percentage of the fraudulent products.