illustra™ AutoSeq™ G-50 columns are specifically designed to remove fluorescent dye-terminators from sequencing reactions prior to analysis on automated sequencers. Effective purification is essential for high quality sequencing results since residual dye-terminators can obscure data. illustra™ AutoSeq™ G-50 consists of MicroSpin™ columns containing Sephadex™ G-50, pre-equilibrated in double-distilled water. This is important because even the small amount of salt in traditional buffers (such as TE buffer) can cause electrophoretic artifacts on salt-sensitive automated sequencers.

• Columns are prepacked with Sephadex™ G-50 DNA Grade F and pre-equilibrated in double-distilled water with 0,05% Kathon CG/ICP Biocide added as a preservative
• Ready to use, enabling sample application to collection of purified product in less than 4 minutes

Effective purification is essential for high-quality sequencing results since residual dye-terminators can obscure data. Columns consist of MicroSpin columns containing Sephadex G-50, pre-equilibrated in double-distilled water. This is important because even the small amount of salt in traditional buffers (such as TE buffer) can cause electrophoretic artifacts on salt-sensitive automated sequencers.

Columns are prepacked with Sephadex G-50 DNA Grade F and pre-equilibrated in double-distilled water with 0.05% Kathon CG/ICP Biocide added as a preservative. Columns that are ready to use enable sample application to collection of purified product in less than 4 min.