Krypton fluorescent protein stain enables sensitive visualisation with fluorescence imagers (520 nm excitation; 580 nm emission) of proteins separated in polyacrylamide gels or transferred to membranes.

• Effective with popular laser-based and filter-based fluorescence imagers
• Linear quantitative range spans three to four orders of magnitude
• Detect as little as 0,25 ng protein with the standard protocol (approx. 2,5 hours); at least as sensitive as other fluorescent stains
• Standard (2,5 hours) and rapid (30 minutes) protocols are significantly faster than procedures of other popular fluorescent stains
• Compatible with proteomics workflows involving downstream mass spectrometry
• Supplied as a 10X solution to save storage space; just dilute with water before use

This fluorescent protein gel stain is for bright yellow-orange fluorescent detection of proteins separated by 1D or 2D polyacrylamide gel electrophoresis (SDS-PAGE). Krypton stain uses a protein-binding dye with fluorescence properties (Ex/Em = 520/580 nm) suitable for visualisation with a variety of standard laser-based and filter-based fluorescence imagers and documentation systems. Proteins are detectable at concentrations greater than 0,25 ng per band in a typical mini-gel lane (greater than 2 ng for the rapid staining protocol). Krypton stain provides high signal intensity, exhibits minimal protein-to-protein variation, has an excellent quantitative dynamic range and is compatible with subsequent destaining and analysis by mass spectrometry.

For best results, detect bands using visible laser-based imagers equipped with a 532 nm laser light source. Although 580 nm filters are optimal for emission, 600 nm-filters are also compatible. Stained gels can be imaged on any platform with the respective excitation and emission filters.

Detection location: In-gel detection
Detection method: Fluorescent
Label or dye: Proprietary mix
Target molecule: Protein