Imperial protein stain is a ready-to-use colorimetric stain formulated with coomassie dye R-250 that delivers consistent nanogram-level detection of proteins in polyacrylamide electrophoresis gels or nitrocellulose membranes.
- Sensitive detects less than 3 ng protein per band with the enhanced protocol (3 hours)
- Time to stain ready-to-use reagent detects less than 6 ng protein per band in just 20 minutes
- High contrast intense purple bands are easier to photograph or scan than typical coomassie blue stains
- Compatibility compatible with downstream mass spectrometry analysis and protein sequencing
- Additional reagents water washes only; no acid-fixative or methanol destaining solutions required
- Stable one year room-temperature stability
The stain is a unique formulation of coomassie brilliant blue R-250 that delivers substantial improvements in protein-staining performance compared to homemade or other commercial stains. Staining results in intensely colored protein bands that are easy to photograph and document with gel imagers. This reagent is one of the most sensitive colorimetric stains available, easily detecting 3 to 6 nanograms of protein per band. The easy-to-follow protocol is flexible to meet demanding time and sensitivity requirements.
Detection location: In-blot detection, In-gel detection
Detection method: Colorimetric
Label or dye: Coomassie
Shelf life: One year
Target Molecule: Protein