The Pierce Glycoprotein Staining Kit is a convenient, fast, and sensitive colorimetric kit for staining glycosylated proteins in polyacrylamide gels or nitrocellulose membranes using the periodic acid-Schiff (PAS) method.

• Specific for glycoproteins - PAS method specifically detects glycosylated proteins with sialic acid and other oxidisable carbohydrate groups
• Protocol - the traditional three-reagent method has been streamlined to achieve optimal staining in less than two hours
• Colorimetric - glycoproteins are detected as magenta bands against a faint pink or colorless gel background
• Qualitative staining - detection limit depends on the extent of glycosylation and protein size and abundance; detects 0,625 ng avidin and 0.16 µg horseradish peroxidase per band
• Controls included - complete kit includes positive and negative control proteins (HRP and STI, respectively)

The kit provides three essential reagents and a complete protocol to specifically stain glycosylated proteins (glycoproteins) that have been separated by polyacrylamide gel electrophoresis (PAGE). A gel or membrane containing separated proteins is treated with a periodate solution, which oxidises cis-diol sugar groups in glycoproteins. The resulting aldehyde groups are detected through the formation of Schiff-base bonds with a reagent that produces magenta bands. Traditionally called the periodic acid-Schiff (PAS) reagent method, this staining technique is made easier by the convenient packaging of essential reagents in one kit with easy-to-follow instructions.

Contents
Sufficient for: 10 mini gels (SDS-PAGE)
• Oxidation reagent, 2,5 g powder to make 250 ml
• Reduction reagent, 1,25 g powder to make 250 ml
• Positive control (Horseradish peroxidase), 1 mg
• Negative control (Soybean trypsin inhibitor), 1 mg
• Glycoprotein stain, 250 ml

Detection location: In-blot detection, in-gel detection
Detection method: Colorimetric
Label or dye: Fuchsin sulfite
Target molecule: Proteins (Glycoproteins)