CM Sephadex™ C-50 Ion Exchange Chromatography Media, Cytiva

Sephadex ion exchangers are produced by introducing functional groups onto the cross-linked dextran matrix.

Weak cation exchanger suitable batch techniques
High binding capacities
Avoid using oxidising agents and dextranase

CM Sephadex A-50 is a weak cation exchanger based on the well documented and well proven Sephadex base matrix. Sephadex ion exchangers are produced by introducing functional groups onto the cross-linked dextran matrix. These groups are attached to glucose units in the matrix by stable ether linkages. Different types of ion exchangers (DEAE, QAE and CM) and two different porosities (A-25/C-25 and A-50/C-50) are available. Different types of ion exchangers (DEAE, QAE and CM) and two different porosities (A-25/C-25 and A-50/C-50) are available.

These groups are attached to glucose units in the matrix by stable ether linkages.

pH range:
6 - 10
Matrix:
Cross-linked dextran
Ligand:
Carboxymethyl
Storage temperature:
4 - 30 °C
Binding capacity:
lgG (Mr 160 000)- 7 mg/ml drained medium), Bovine COHb (Mr 69 000)- 140 mg/ml drained medium), Ribonuclease (Mr 13 700)- 120 mg/ml drained medium)
Total ionic capacity:
4 - 5 mmol/g
Description:
CM Sephadex™ C-50