DEAE Sephadex™ A-50 Ion Exchange Chromatography Media, Cytiva
Supplier: Cytiva
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About this item
DEAE Sephadex™ A-25 and DEAE Sephadex™ A-50 are weak anion exchangers. Sephadex™ ion exchangers are produced by introducing functional groups onto the cross-linked dextran matrix. These groups are attached to glucose units in the matrix by stable ether linkages.
- Weak anion exchanger suitable for batch techniques
- High binding capacities
- Well-proven and widely used
- Stable to all commonly used aqueous buffers additives such as 8 M urea and 6 M guanidine hydrochloride
These groups are attached to glucose units in the matrix by stable ether linkages. Different types of ion exchangers (DEAE, QAE and CM) and two different porosities (A-25/C-25 and A-50/C-50) are available.
Sephadex ion exchangers are produced by introducing functional groups onto the cross-linked dextran matrix.
Specifications
- pH range:2 - 9
- Matrix:Cross-linked dextran
- Ligand:Diethylaminoethyl
- Storage temperature:4 - 30 °C
- Binding capacity:Thyroglobulin (Mr 669 000)- 2 mg/ml drained resin), HSA (Mr 68 000)- 110 mg/ml drained resin), α-lactalbumin (Mr 14 300)- 50 mg/ml drained resin)
- Total ionic capacity:3-4 mmol/g
- Description:DEAE Sephadex™ A-50