For the quantification of PGIM in urine and other sample matrices.

• Measure PGIMs in urine
• Measure PGIM down to 120 pg/ml
• Convenient overnight format

Prostaglandin I (Prostaglandin I₂; PGI₂) is a potent vasodilator and inhibitor of platelet aggregation that is formed from arachidonic acid primarily in the vascular endothelium and renal cortex by sequential activities of cyclooxygenase (COX) and PGI₂ synthase. It is non-enzymatically hydrated to 6-keto PGF_1α (t_½ = 2 to 3 minutes), and then quickly converted to numerous downstream metabolites, collectively known as prostaglandin I metabolites or PGIM, (t_½= 30 minutes). Although 6-keto PGF_1α is commonly measured in plasma and urine as an estimate of PGI₂ synthesis, it should be noted that there may be more than one source of PGI₂ in these samples. For instance, urinary concentrations of 6-keto PGF_1α are confounded by the fact that only a portion originates from plasma and the remainder is produced by the kidney. These problems are circumvented by measuring downstream PGIM rather than 6-keto PGF_1α as an indicator of systemic PGI₂ production. Cayman's PGIM ELISA Kit is a competitive assay that can be used for quantification of PGIM in urine and other sample matrices. The assay has a range from 39 to 5000 pg/ml and a sensitivity (80% B/B₀) of approximately 120 pg/ml.

Contents: Antibody, tracer, standard, buffer, polysorbate 20, reagent, dyes, plate, and cover sheet.