VWR®, Trypsin-EDTA solutions
About this item
VWR® Trypsin-EDTA solutions are used in cell culture to remove adherent cells from a culture surface. Trypsin is a porcine pancreas-derived enzyme that is commonly used for the dissociation and disaggregation of anchorage-dependent mammalian cells and tissues. The concentration of trypsin necessary to separate cells from their substrate is dependent primarily on the cell type and the age of the culture. EDTA, a chelating agent, enhances enzymatic activity by removing calcium and magnesium ions. These ions obscure the peptide bonds on which trypsin acts as well as enhancing cell to cell adhesion.
- Trypsin EDTA solutions exist in different buffers and concentrations
- Trypsin 0,25% - EDTA in HBSS without calcium, without magnesium, with phenol red
- Trypsin EDTA 1X in PBS without calcium, without magnesium without phenol red
- Trypsin EDTA 1X without calcium, without magnesium with phenol red
- Trypsin-EDTA 10X
After treatment, trypsin activity can be stopped by adding a serum media mixture or a trypsin inhibitor.
4 Options Available Below
Product Details & Documents
VWR® Trypsin-EDTA solutions are used in cell culture to remove adherent cells from a culture surface. Trypsin is a porcine pancreas-derived enzyme that is commonly used for the dissociation and disaggregation of anchorage-dependent mammalian cells and tissues. The concentration of trypsin necessary to separate cells from their substrate is dependent primarily on the cell type and the age of the culture. EDTA, a chelating agent, enhances enzymatic activity by removing calcium and magnesium ions. These ions obscure the peptide bonds on which trypsin acts as well as enhancing cell to cell adhesion.
- Trypsin EDTA solutions exist in different buffers and concentrations
- Trypsin 0,25% - EDTA in HBSS without calcium, without magnesium, with phenol red
- Trypsin EDTA 1X in PBS without calcium, without magnesium without phenol red
- Trypsin EDTA 1X without calcium, without magnesium with phenol red
- Trypsin-EDTA 10X
After treatment, trypsin activity can be stopped by adding a serum media mixture or a trypsin inhibitor.
Certificates
Search by Lot NumberSpecifications for Product Family
Specifications
Specifications
- Catalog No:
- 392-0435
- 392-0436
- 392-0437
- 392-0459
- Sterility:
- Sterile
- Sterile
- Sterile
- Sterile
Specifications
Product Family Options
Product Information
- FormPkAvailabilityPrice
- 392-0435Trypsin-EDTA 1X without Ca/Mg, with Phenol RedTrypsin-EDTA 1X without Ca/Mg, with Phenol RedLiquid100 mlLog in for availability1 * 100 mL11,10 €Specifications:
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Specifications
Cat. No.392-0435SterilitySterile - 392-0436Trypsin 0,25% - EDTA in HBSS without Ca/MgSpecifications:
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Specifications
Cat. No.392-0436SterilitySterile - 392-0437Trypsin-EDTA 1X in PBS without Ca/Mg, without Phenol RedTrypsin-EDTA 1X in PBS without Ca/Mg, without Phenol RedLiquid100 mlLog in for availability1 * 100 mL10,80 €Specifications:
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Specifications
Cat. No.392-0437SterilitySterile - 392-0459Trypsin-EDTA 10XSpecifications:
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Specifications
Cat. No.392-0459SterilitySterile
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