illustra™ tissue and cells genomicPrep Mini Spin Kit is designed for the rapid extraction and purification of high quality genomic DNA from a variety of animal tissues and mammalian cell cultures. The kit can be used for several downstream applications including restriction enzyme analysis, ligation, cloning, DNA sequencing and PCR.

• Rapid procedure produces high molecular weight gDNA with minimal shearing
• Capable of purifying up to 1,5 μg/mg of tissue from an input tissue sample range of 5 to 50 mg, and up to 40 μg of genomic DNA per 5×10⁶ cells depending on cell type
• Total DNA extraction time approximately 90 minutes for tissues and 45 minutes for cells
• Produces high-quality genomic DNA with high molecular weight and less degradation
• Verified in common downstream applications

Human and animal genomic DNA is found in the chromosomes and it is much longer and higher in molecular weight than plasmid DNA. Genomic DNA is the code for the genome, and this is what is needed to do genome sequencing.

For sequencing it is desirable that the genomic DNA extraction technique used allows the collection of intact genomic DNA in the longest fragments possible. A kit format can help standardize the procedures used to prepare tissue and cell genomic DNA samples for analysis.

To minimize the incidence of DNA shearing during extraction, Tissue and cells genomicPrep Mini Spin Kit includes a lysis solution with proteinase K to gently release genomic DNA from tissues or cells, including human genomic DNA.

The kit can be used for DNA isolation from diverse tissue types such as liver, kidney, and mouse tails with yields ranging from 0.5 to 1.5 μg of genomic DNA per mg of tissue. The kit consistently generates purified genomic DNA with optical density A260/A280 readings of 1.8 or higher.

The gDNA produced can then be further analyzed in PCR, for SNP genotyping, and next generation sequencing (NGS) projects.