Kits for increasing immunofluorescence sensitivity using HRP-catalysed tyramide signal amplification with the choice of next-generation fluorescent CF® dyes or biotin.

• Increase sensitivity by up to 100-fold
• Use lower primary antibody concentrations
• Boost signal in challenging samples, like human FFPE tissues
• Choose from six bright and photostable CF® dyes or biotin

Tyramide amplification, sometimes called Catalysed Reporter Deposition (CARD), is an enzyme-mediated detection method that uses the catalytic activity of horseradish peroxidase (HRP) to generate high density labelling of a target protein or nucleic acid sequence in situ. The tyramide amplification method has been reported to increase the sensitivity by up to 100-fold compared to conventional avidin-biotin detection. Multiple rounds of tyramide signal amplification can be performed for multiplex detection.

The HRP goat anti-rabbit antibody provided in the kits has been adsorbed against human, mouse, and rat serum to minimise cross-reactivity. The kits include choice of six CF® dye tyramides or biotin tyramide. CF® dyes are line of next-generation fluorescent dyes, with advantages in brightness and photostability compared to other dyes.