The Biosensis Alpha-Synuclein Rapid™ ELISA kit is a sandwich ELISA that allows the quantification of human alpha-synuclein in less than 4 hours in human citrate-plasma, serum and CSF only if used as directed.

• Complete ELISA kit with reagents and detailed protocols
• Fast assay (<4 hours) without compromising accuracy and precision
• Validated for human blood and CSF samples
• Refer to the kit protocol for specific use instructions for blood and CSF application

This ELISA kit consists of a pre-coated sheep polyclonal anti-alpha-synuclein (aa: 116-131) capture antibody, a biotinylated mouse monoclonal anti-alpha-synuclein detection antibody (aa: 61-95) and horseradish peroxidase (HRP)-conjugated streptavidin. The addition of a substrate (3,3',5,5'-tetramethylbenzidine, TMB) yields a colored reaction product which is directly proportional to the concentration of alpha-synuclein present in samples and protein standards. A human alpha-synuclein positive control (QC sample) is provided to assure consistent assay performance.

Specificity: Human alpha-synuclein. Detection of individual alpha-synuclein isoforms has not yet been investigated. However, due to the nature of the assay antibodies, this ELISA kit is not expected to discriminate between various alpha-synuclein isoforms (monomeric, oligomeric, phosphorylated).

Cross-reactivity with beta- and gamma-synuclein was calculated based on increase or decrease of apparent endogenous α-synuclein concentrations when spiking beta- and gamma-synuclein into two human plasma samples. This ELISA shows very little cross-reactivity of 2,3% or less for beta- and gamma-synuclein.