Mix-n-Stain™ Biotin antibody labelling kits utilise a technology that allows rapid antibody labelling with biotin without a purification step.
- Tolerates many common buffer components and antibody stabilisers
Mix-n-Stain™ Antibody Labeling Kits feature Biotium's line of CF™ dyes, which offer advantages in brighness and/or photostability compared to Alexa Fluor® dyes and other similar commercial dyes. A large selection of CF™ dye colors spanning the visible and near-infrared spectra offers flexibility for labeling primary antibodies with dye colors that are optimal for your instrument detection settings for multi-color detection. See the CF™ Dye Reference Table under the Datasheets tab for more information.
CF™ dye technologies are covered by pending US and international patents. Mix-n-Stain™ and CF™ are trademarks of Biotium, Inc. AlexaFluor® is a registered trademark of Molecular Probes, Inc.
Simply mix your antibody with the buffer and premeasured aliquot of CF™ dye provided in the kit, a step that takes less than 30 seconds of hands-on time. After a 30 minute incubation, your fluorescent antibody conjugate is ready to use, with no purification step. There is no need to calculate how much dye you should use; just select the approptriate kit size based on the ug amount of antibody you wish to label for optimal results. Moreover, the labeling reaction can tolerate the presence of common stabilisers such as sodium azide and low levels of Tris, glycerol, BSA or gelatin. An ultrafiltration column provided in the kit can be used to remove Tris, glycine, or glycerol if the concentration in your antibody solution exceeds the limits specified in the labeling protocol. See the product protocol under the Datasheets tab for more information.