- Antibody type:Primary
- Antigen name:Ki-67
- Antigen symbol:KI67
- Clonality:Monoclonal
- Clone:6B4
- Conjugation:Unconjugated
- Host:Mouse
- ImmunoChemistry:Yes
- ImmunoFluorescence:Yes
- Isotype:IgG1
- Reactivity:Human
- Western blot:Yes
- Format:Purified IgG
- Form:Lyophilized
- Antigen synonyms:Proliferation marker protein Ki-67|Antigen KI-67|Antigen Ki67|Antigen identified by monoclonal antibody Ki-67
- UniProtKB:Uniprot # P46013
- Storage temperature:Store lyophilised antibody at 2-8 °C. After reconstitution divide into aliquots and store at –20 °C for long-term storage. Store at 2-8 °C short-term (up to 4 weeks) with an appropriate antibacterial agent. Avoid repetitive freeze/thaw cycles.
- Shipping temperature:Ambient
- Immunogen:Recombinant human Ki-67 protein (amino acids 1,111-1,490) expressed in and purified from E. coli.
- Purification:Affinity purified
- Size:100 µg
- Pk:100 µG
Required to maintain individual mitotic chromosomes dispersed in the cytoplasm following nuclear envelope disassembly (PubMed:27362226). Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the chromosome surface (PubMed:27362226). Prevents chromosomes from collapsing into a single chromatin mass by forming a steric and electrostatic charge barrier: the protein has a high net electrical charge and acts as a surfactant, dispersing chromosomes and enabling independent chromosome motility (PubMed:27362226). Binds DNA, with a preference for supercoiled DNA and AT-rich DNA (PubMed:10878551). Does not contribute to the internal structure of mitotic chromosomes (By similarity). May play a role in chromatin organization (PubMed:24867636). It is however unclear whether it plays a direct role in chromatin organization or whether it is an indirect consequence of its function in maintaining mitotic chromosomes dispersed (Probable). Ref: uniprot.org
Western blotting (1:1,000-1:5,000) and Immunocytochemistry (1:2,000-1:5,000). Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Type: Primary
Antigen: KI67
Clonality: Monoclonal
Clone: 6B4
Conjugation: Unconjugated
Epitope:
Host: Mouse
Isotype: IgG1
Reactivity: Human