Phire™ Tissue Direct PCR Master Mix has been developed for amplification of DNA directly from a wide variety of tissues obtained from mice, human, fish, birds and insects. The master mix containing Phire™ Hot Start II DNA polymerase is specially formulated to perform PCR in the presence of different animal tissue-derived inhibitors, such as collagen, melanin and eumelanin (hair, skin) or myoglobin (muscle).
- Sample is added directly to PCR reaction, therefore there is no need for time-consuming and expensive DNA purification steps
- Dilution & storage protocol available which allows tens of PCR reactions from one tiny sample and allows re-testing
- Specially engineered Phire™ DNA polymerase allows extremely short PCR protocol times
- Master Mix format with premixed gel loading dye minimises possibility of cross-contamination, reduces sample handling and allows direct loading on gel
The kit also includes Thermo Scientific™ DNARelease Additive, which can be used to improve the release of DNA from difficult tissues.
The optimal annealing temperature for Phire™ DNA Polymerases may differ significantly from that of Taq-based polymerases.
Applications include genotyping, transgene detection, knockout analysis and sequencing.