Pierce™ Protein L coated plates provide an alternative to direct, passive adsorption methods for immobilising antibodies for ELISA and other plate-based assay techniques.

• Retain antibody activity, which can be lost when antibodies are immobilised by passive adsorption
• Orient antibodies for maximum antigen-binding capacity
• Immobilise antibodies for plate assays without prior purification
• Ensure minimal variation (<5% well-to-well) from consistent coating
• Plates are pre-blocked with SuperBlock™ blocking buffer to reduce non specific binding
• Bind 2 to 5 pmol of antibody per well (based on tests with rabbit IgG)
• Binding Property: Standard Capacity, Affinity; Detection Method: Colorimetric

Protein L binds to immunoglobulin kappa light chains without interfering with the antigen-binding site. Protein L binds to all classes of Ig (ie. IgG, IgM, IgA, IgE and IgD). Protein L also binds single chain variable fragments (scFv) and Fab fragments.