The HisPur™ Ni-NTA Spin Purification Kit provides a fast, easy to use format for routine affinity purification of His-tagged fusion proteins and contains 0,2-ml HisPur™ Ni-NTA spin columns, as well as buffers required to purify polyhistidine-tagged proteins.
- Purifies high levels of overexpressed His-tagged fusion proteins from bacterial lysates
- Purify proteins using native or denaturing conditions
- High capacity
- Compatible
- Cost-effective
- Easy to use
- Flexible
HisPur™ Ni-NTA Resin effectively purifies high levels of overexpressed His-tagged fusion proteins from bacterial lysates, such as those that are attained with Thermo Scientific B-PER Bacterial Protein Extraction Reagents. The resin performs well in batch-binding and spin-column procedures at a variety of scales. Performance equals or exceeds popular Ni-NTA resins from other suppliers. HisPur™ Ni-NTA Resin is a high-quality, stable and resilient affinity support. Tests confirm that no decrease in performance occurs after at least five repeated uses. These data indicated that the resin is highly resistant to structural degradation or nickel ion leaching during normal use.
HisPur™ Ni-NTA resin is a high-capacity, high-performance nickel-IMAC resin for routine affinity purification of His-tagged fusion proteins.