Pierce™ Maleic activated plates allow attachment of amine-containing peptides and other molecules to microplate wells for use in binding assays. The plates are useful for immobilising peptides and other ligands that do not coat efficiently by passive adsorption. Clear plates are suitable for ELISA and other assays. White plates are designed for chemiluminescent assays.

• Spontaneously react with primary amines
• Direct, simple approach to covalently attach molecules to the well surface of an EIA plate
• Maleic anhydride maintains its integrity and availability for coupling for months under dry, room temperature conditions
• Binding Property: Standard Capacity, Covalent
• Detection Method : Colorimetric method
• Surface Treatment: Maleic Anhydride

Activation level: 200 µl/well
Binding capacity: ~125 pmol biotin pentylamine (328 Da)/well

Coating reactions are best performed at pH 8 to 9, and the reacted plates are best used at pH greater than 7 for ELISA and other methods.