The DyLight™ Specialty Dyes are fluorescent dyes with varying spectral characteristics, degrees of sulfonation, charges and hydrophobicities. These dyes are offered in their amine-reactive forms and are mainly derivatized with N-hydroxysuccinimide (NHS) esters, the simplest and most commonly used reactive group for labeling proteins. The large selection of near infrared (NIR) and infrared (IR) dyes may be used for in vivo and near-IR fluorescence (NIRF) applications.

• NHS ester reactive group—allows immediate labeling of antibodies, proteins, peptides and other amine-containing molecules through amide bond formation
• Water solubility—1 sulfonate group increases the solubility of this dye over over nonsulfonated dyes

DyLight Blue-emitting Dye is a labeling agent that provides bright fluorescence detection for imaging and flow cytometry. Dyes that contain a greater number of negatively charged sulfonates generally will have greater water solubility than dyes with fewer sulfonates. More hydrophobic dyes often provide better cell penetration in vivo, while more hydrophilic dyes have less nonspecific binding potential. This dye contains 1 sulfonate for increased solubility and an amine-reactive NHS ester for simple modification of antibodies, proteins, peptides, or other biomolecules through amide bond formation.