Clone OX-42 recognises the rat equivalent of human CD11b. CD11b is a single-pass type I membrane protein that belongs to the integrin alpha chain family. CD11b is predominantly expressed in monocytes and granulocytes and is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles (Ref: SWISSPROT). CD11b is also frequently used as a microglial marker allowing to distinguish between quiescent and activated microglia based on the intensity of CD11b staining.

Detects three polypeptides of 160 kDa, 103 kDa and 95 kDa and a fainter band may also be seen at 133 kDa under non-reducing conditions. Under reducing conditions two major peptides of 163 kDa and 100 kDa and a minor 135 kDa peptide are seen.
Application Information:
Immunohistochemistry (Formalin/Paraformaldehyde-fixed paraffin-embedded sections): 1/25 - 1/200; Immunocytochemistry: 1/200. Incubate overnight; ELISA: 1/100 - 1/3200. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.

Type: Primary
Antigen: Macrophages
Clonality: Monoclonal
Clone: OX42
Conjugation: Unconjugated
Epitope:
Host: Mouse
Isotype:
Reactivity: Rat