The femtoELISA™ kits utilise a non animal protein blocker, NAP-BLOCKER™ that minimises cross-reactivity with researcher's antigens and antibodies. For the detection of HRP, an improved, ultra sensitive, non volatile, stable colorimetric substrate based on tetramethyl benzidine (TMB) is supplied. Reagent does not require hydrogen peroxide that can have detrimental effects on many biological factors, which may ultimately affect the assays.

For the detection of alkaline phosphatase, a p-nitrophenylphosphate (pNPP) based substrate is supplied that has superior stability compared to commonly used pNPP tablets and solutions. The improved stability ensures minimal background Ø over longer periods compared to normal pNPP substrates. Our highly rapid AP substrate has superior sensitivity and requires no preparation time.