Agarose and Acrylamide

Agarose and acrylamide are the most commonly used electrophoresis gels for their versatility with buffers and ability to generate reproducible results. Agarose typically runs horizontal tests to resolve large DNA fragments while acrylamide runs vertical separations for shorter nucleic acids. Easy to handle, the gels are chosen based on the high resolution desired and the samples. The concentration or ratio of the agarose and acrylamide will determine the overall working porosity.

VWR® Acrylamide-Bis Solution (37,5:1) 30% Aqueous Solution, Ultra Pure Grade

Supplier: VWR Chemicals

Electrophoresis Gels, Acryl/Bis™, Acrylamide-Bis solution (37,5:1) 30% aqueous solution

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GelGreen® Agarose LE

Supplier: Biotium

Ultrapure molecular biology grade agarose pre-coated with GelGreen® Nucleic acid gel stain for convenient and safer preparation of precast gels.

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VWR® Acrylamide and Bis-Acrylamide

Supplier: VWR Chemicals

An extensive line of acrylamide and bis-acrylamide pre-weighed powder blends, pre-mixed stock solutions and ready to use solutions for customised polyacrylamide gel electrophoresis of proteins and PAGE of nucleic acids.

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Peqlab peqGOLD, Agarose, Universal

Supplier: VWR Chemicals

High quality standard agarose for routine analysis with broad separation range (0,05 to 50 kbp). Ideal for blotting applications due to high gel strength. Free from RNases, DNases and enzyme inhibitors.

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VWR®, Agarose RA™

Supplier: VWR Chemicals

Agarose RA™ is a general application agarose that provides good resolution and is cost effective for high volume users. It was developed specifically for quick checks of PCR products, plasmids preps, screening and cloning.

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VWR® Acryl/Bis™ Acrylamine/Bis-Acrylamine

Supplier: VWR Chemicals

A 40% (w/v) solution of ultra pure acrylamide (38,96%) and bis-acrylamide (1,04%) at a final ratio of 37.5:1.

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Agarose LE, ultra-pure molecular bology grade

Supplier: Biotium

High performance agarose for nucleic acid electrophoresis (analytical or preparative) and blotting. Validated for use with GelRed®, GelGreen®, and DNAzure® gel stains.

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VWR®, Agarose I™

Supplier: VWR Chemicals

Agarose I™ is a standard melting/gelling agarose, suitable for routine nucleic acid analytical/preparative applications (250 bp to 22 kb).

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Acryl/Bis solution (30%) 29:1, ultrapure

Supplier: VWR Chemicals

Electrophoresis Gels, Acryl/Bis™, Acryl/Bis solution (30%) 29:1

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Agarose II, low melting point, Pierce™

Supplier: Thermo Fisher Scientific

Agarose II (low melt) is a highly purified and carefully blended formulation that provides uniform lot-to-lot consistency for preparation of electrophoresis gels that require a lower melting temperature.

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Melt & Pour UltraSpec-Agarose™

Supplier: EDVOTEK

0.8% UltraSpec-Agarose™ prepared with TAE buffer. Simply melt, cool and pour.

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UltraSpec-Agarose™, DNA electrophoresis grade

Supplier: EDVOTEK

Electrophoresis Gels, UltraSpec-Agarose™

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Agarose I, Pierce™

Supplier: Thermo Fisher Scientific

Agarose I (molecular biology grade) is a highly purified and carefully blended formulation that provides uniform lot-to-lot consistency for preparation of electrophoresis gels.

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Agarose, Molecular Biology Grade, Ultrapure

Supplier: Thermo Fisher Scientific

This agarose is a granular, free-flowing powder.

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Acrylamide/ Bis-Acrylamide

Supplier: G-Biosciences

Acrylamide (electrophoresis grade) is supplied as a powder or a 40% solution in ultrapure water. Bisacrylamide (Bis (N,N’-methylenebisacrylamide)) (electrophoresis grade) is supplied as a powder or a 2% solution in ultrapure water.

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Agarose, low gelling temperature

Supplier: Thermo Fisher Scientific

Agarose is useful in separation of nucleic acids electrophoretically because agarose gels have larger pore sizes than cross linked acrylamide gels at low concentrations and in chromatographic separations by either beading the agarose or cross-linking it. Agarose gels are also used in immunoelectrophoresis (IEP) and double-diffusion Ouchterlony plates to demonstrate antibody-antigen reactions.