Protein Extraction Reagents

Efficient cell lysis and maximum protein extraction yields are vital to high-quality recombinant protein purification. The protease inhibitor cocktails are for lysing mammalian cells, buffers that solubilize plasma and intracellular membranes, break weak intermolecular bonds, and solubilize most proteins. Making procedures easier to perform, these protein extraction reagents can also be used to isolate proteins from both the cytoplasmic and nuclear fractions of cultured cells.

Detergent removal system, DetergentOUT™ Tween®

Supplier: G-Biosciences

DetergentOUT™ Tween® is a spin column format detergent removal resin for polysorbate or Tween® detergents or surfactants.

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Trypsin Antigen Retrieval Kit, Abcam

Supplier: Abcam

Trypsin Antigen Retrieval Kit ab259256 is used for enzyme-based antigen retrieval.

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Trypsin Antigen Retrieval Solution, Abcam

Supplier: Abcam

Trypsin-based antigen retrieval solution for Protease-Induced Epitope Retrieval (PIER) (ab970).

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Universal nuclease for cell lysis, Pierce™

Supplier: Thermo Fisher Scientific

The Pierce™ Universal Nuclease for Cell Lysis is ideal for a wide variety of applications where complete digestion of nucleic acids is needed when preparing cell lysates.

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HistoReveal (15ml), Abcam

Supplier: Abcam

Proteolytic antigen retrieval (PIER) reagent HistoReveal (ab103720).

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Pierce™ EndoProteinase Lys-C Protease, MS Grade

Supplier: Thermo Fisher Scientific

Endoproteinase Lys-C protease is a highly purified native endoproteinase validated for maximum activity and stability in proteomic applications.

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Detergent removal systems, OrgoSol DetergentOUT™

Supplier: G-Biosciences

OrgoSol DetergentOUT™ is suitable for removal of detergents from protein solutions, including hydrophobic protein solutions. Compatible with all detergent types, its performance is not dependent on the concentration of detergents in the solution. It is highly flexible and can be used to process small and large sample volumes.

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Immobilized pepsin, Pierce™

Supplier: Thermo Fisher Scientific

Immobilised Pepsin agarose consists of an acidic endopeptidase that has been immobilised onto beaded agarose resin to enable generation and purification of Fab and F(ab')2 fragments from antibodies.

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Adenine deaminase

Supplier: G-Biosciences

Adenine deaminase converts adenine to hypoxanthine. It is a highly purified recombinant enzyme protein supplied at a 1 µM concentration.

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Pierce™ Digestion Indicator for Mass Spectrometry, Thermo Scientific

Supplier: Thermo Fisher Scientific

The Digestion Indicator for Mass Spectrometry is a non-mammalian recombinant protein (26kDa) with five signature peptides for use in determining the digestion efficiency and reproducibility across multiple samples.

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Detergent removal spin column kit, HiPPR™

Supplier: Thermo Fisher Scientific

The HiPPR™ Detergent Removal Spin Column Kit improves mass spectrometry results by efficiently removing detergents from 25 to 200 µl samples with low protein or peptide concentrations.

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Pierce™ Detergent Removal Spin Columns and Resin

Supplier: Thermo Fisher Scientific

Pierce™ Detergent removal resin enables removal of commonly used ionic, non-ionic and zwitterionic detergents from protein/peptide digest solutions.

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Immobilised papain, Pierce™

Supplier: Thermo Fisher Scientific

Pierce™ Immobilised Papain consists of a cysteine-endopeptidase that has been immobilised onto beaded agarose resin to enable generation and purification of Fab fragments from antibodies.

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Ovalbumin (in PBS), Imject™

Supplier: Thermo Fisher Scientific

Ovalbumin (in PBS), Imject™

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Immobilised Papain

Supplier: G-Biosciences

A cysteine protease enzyme (EC 3.4.22.2) immobilised on 4% agarose, cleaves Immunoglobulin G (IgG) antibody molecules in the hinge region, generating three ~50 kDa fragments; two Fab domains and a Fc domain. The papain-digested antibody is unable to promote agglutination, precipitation, opsonisation, and lysis.

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Micrococcal nuclease solution (≥100 U/µl)

Supplier: Thermo Fisher Scientific

This Micrococcal Nuclease (MNase) is suitable for removing nucleic acids from cell lysates, releasing chromatin-bound proteins and shearing chromatin for use in chromatin immunoprecipitation (ChIP) experiments.