You searched for: Nucleic Acid Reagents
Nucleic acid reagents form the foundation of genetic analysis and molecular biology, playing a pivotal role from cloning to gene expression studies. Delve into our collection featuring high-fidelity enzymes for end-point PCR, advanced reagents for isothermal amplification, and comprehensive kits for nucleic acid purification. For precision in quantitative assays, explore our selection of qPCR and RT-qPCR kits, primed for unparalleled accuracy. Our portfolio also includes next-generation sequencing reagents, offering cutting-edge solutions for genomic sequencing and personalized medicine advancements.
NED Dye qPCR Calibration Solution *10000X*
Supplier: AAT BIOQUEST
NED dye qPCR Calibration Solution supplied as a 10000X concentrate is compatible with a wide variety of qPCR instruments, including the ABI7500 Fast, QuantStudio™, and ViiA™ seven systems.
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JUN Dye qPCR Calibration Solution *10000X*
Supplier: AAT BIOQUEST
JUN dye qPCR Calibration Solution supplied as a 10000X concentrate is compatible with a wide variety of qPCR instruments, including the ABI7500 Fast, QuantStudio™, and ViiA™ 7 systems.
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VIC Dye qPCR Calibration Solution *10000X*
Supplier: AAT BIOQUEST
VIC dye qPCR Calibration Solution supplied as a 10000X concentrate is compatible with a wide variety of qPCR instruments, including the ABI7500 Fast, QuantStudio™, and ViiA™ 7 systems.
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Cy5 Dye qPCR Calibration Solution *10000X*
Supplier: AAT BIOQUEST
Real-time thermal cyclers require calibration to generate a spectral profile for the fluorescence signal.
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TAQuest™ FAST qPCR Master Mix for TaqMan Probes *No ROX*
Supplier: AAT BIOQUEST
TAQuest™ FAST qPCR Master mix for TaqMan probes is a ready-to-use 2X solution optimised for qPCR and two-step RT-qPCR well suited to use for TaqMan gene expression assays.
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TAQuest™ qPCR Master Mix with Helixyte™ Green *No ROX*
Supplier: AAT BIOQUEST
TAQuest™ qPCR Master mix with Helixyte™ green is a ready-to-use 2X solution optimised for qPCR and two-step RT-qPCR.
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TAQuest™ qPCR Master Mix with Helixyte™ Green *Low ROX*
Supplier: AAT BIOQUEST
TAQuest™ qPCR Master mix with Helixyte™ green is a ready-to-use 2X solution optimised for qPCR and two-step RT-qPCR.
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TAQuest™ FAST qPCR Master Mix with Helixyte™ Green *Low ROX*
Supplier: AAT BIOQUEST
TAQuest™ FAST qPCR Master mix with Helixyte™ green is a ready-to-use 2X solution optimised for qPCR and two-step RT-qPCR.
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SYBR Dye qPCR Calibration Solution *10000X*
Supplier: AAT BIOQUEST
SYBR dye qPCR Calibration solution supplied as a 10000X concentrate is compatible with a wide variety of qPCR instruments, including the ABI7500 Fast, QuantStudio™, and ViiA™ seven systems.
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TAQuest™ qPCR Master Mix for TaqMan Probes *Low ROX*
Supplier: AAT BIOQUEST
TAQuest™ qPCR Master mix for TaqMan probes is a ready-to-use 2X solution optimised for qPCR and two-step RT-qPCR compatible with TaqMan gene expression assays.
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TAQuest™ qPCR Master Mix for TaqMan Probes *High ROX*
Supplier: AAT BIOQUEST
TAQuest™ qPCR Master mix for TaqMan probes is a ready-to-use 2X solution optimised for qPCR and two-step RT-qPCR compatible with TaqMan gene expression assays.
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TAQuest™ FAST qPCR Master Mix for TaqMan Probes *High ROX*
Supplier: AAT BIOQUEST
TAQuest™ FAST qPCR Master mix for TaqMan probes is a ready-to-use 2X solution optimised for qPCR and two-step RT-qPCR well suited to use for TaqMan gene expression assays.
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TAQuest™ qPCR Master Mix with Helixyte™ Green *High ROX*
Supplier: AAT BIOQUEST
TAQuest™ qPCR Master mix with Helixyte™ green is a ready-to-use 2X solution optimised for qPCR and two-step RT-qPCR.
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TAQuest™ qPCR Master Mix for TaqMan Probes *No ROX*
Supplier: AAT BIOQUEST
TAQuest™ qPCR Master mix for TaqMan probes is a ready-to-use 2X solution optimised for qPCR and two-step RT-qPCR compatible with TaqMan gene expression assays.
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TAMRA Dye qPCR Calibration Solution *10000X*
Supplier: AAT BIOQUEST
TAMRA dye qPCR Calibration Solution supplied as a 10000X concentrate is compatible with a wide variety of qPCR instruments, including the ABI7500 Fast, QuantStudio™, and ViiA™ 7 systems
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dNTP solutions, illustra™
Supplier: Cytiva
illustra™ dNTPs are high purity solutions for enzymatically sensitive applications, such as nucleic acid amplification, sequencing, labelling, mutagenesis, cDNA synthesis and expression profiling.
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TAQuest™ FAST qPCR Master Mix with Helixyte™ Green *No ROX*
Supplier: AAT BIOQUEST
TAQuest™ FAST qPCR Master mix with Helixyte™ green is a ready-to-use 2X solution optimised for qPCR and two-step RT-qPCR.
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TAQuest™ FAST qPCR Master Mix with Helixyte™ Green *High ROX*
Supplier: AAT BIOQUEST
TAQuest™ FAST qPCR Master mix with Helixyte™ green is a ready-to-use 2X solution optimised for qPCR and two-step RT-qPCR.
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Luminaris color HiGreen qPCR master mix
Supplier: Thermo Fisher Scientific
Luminaris Color HiGreen qPCR Master Mixes are universal ready to use solutions optimised for qPCR and two-step RT-qPCR. The master mixes include hot start Taq DNA polymerase, uracil-DNA glycosylase (UDG) and dNTPs in an optimised PCR buffer. Only the template and primers need to be added. The SYBR® Green I intercalating dye allows for DNA detection and analysis without using sequence-specific probes.
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DreamTaq™ Hot Start DNA polymerase
Supplier: Thermo Fisher Scientific
Thermo Scientific™ DreamTaq™ Hot Start DNA Polymerase is an enhanced hot start Taq DNA polymerase that ensures higher PCR specificity, sensitivity and yields compared to conventional hot start Taq DNA polymerases. DreamTaq™ Hot Start DNA Polymerase uses antibody based inhibition of DNA polymerase activity at ambient temperatures, thus preventing the amplification of non-specific products prior to the amplification step.
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Phusion® Green High-Fidelity DNA Polymerase
Supplier: FINNZYMES REAGENTS
Phusion® Green High-Fidelity DNA Polymerase is a combination of Phusion® DNA Polymerase and 5× Phusion® Green buffers. The buffers include a density reagent and two tracking dyes for direct loading of PCR products on a gel. The coloured buffer does not interfere with excellent performance of Phusion® DNA Polymerases and is compatible with downstream applications such as DNA sequencing, ligation and restriction digestion.
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illustra™ PCR nucleotide mix dNTP set
Supplier: Cytiva
PCR nucleotide mix dNTP set (2 mM, 10 mM, or 25 mM each A,C,G,T) for amplification, dideoxy sequencing, labelling, mutagenesis, cDNA synthesis, and expression profiling.
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dNTP set, MP Biomedicals
Supplier: MP Biomedicals
Set consists of 100 mM aqueous solutions of dATP, dCTP, dGTP and dTTP each supplied in a separate vial.
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KOD Hot start master mix
Supplier: Merck Millipore (Novagen)
Premixed 2X KOD Hot Start PCR components for convenience and reproducibility.
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Maxima™ Probe qPCR Master Mix (2X)
Supplier: Thermo Fisher Scientific
The Maxima™ Probe qPCR Master Mix (2X) is a ready to use solution optimised for quantitative real time PCR and two-step real time RT-PCR on most real time PCR machines. The master mix includes Maxima™ hot start Taq DNA polymerase and dNTPs in an optimised PCR buffer. dUTP is included in the mix for optional carryover contamination control using uracil-DNA glycosylase (UDG). The use of Maxima™ probe qPCR master mix (2X) in real time PCR ensures reproducible, sensitive and specific quantification of genomic, plasmid, viral and cDNA templates.
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Luminaris color Probe qPCR master mixes
Supplier: Thermo Fisher Scientific
Luminaris Color Probe qPCR Master Mixes are universal ready-to-use solutions optimised for qPCR and two-step RT-qPCR. The master mixes include hot start Taq DNA polymerase, uracil-DNA glycosylase (UDG) and dNTPs in an optimised PCR buffer. Only the template, primers and probe need to be added.
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Phire® Green Hot Start II DNA Polymerase
Supplier: FINNZYMES REAGENTS
Phire® Hot Start II DNA Polymerase is faster, extremely robust, and capable of amplifying long DNA fragments with high yields. Phire® Hot Start II DNA Polymerase incorporates a dsDNA-binding domain which allows short extension times (10 to 15 s/kb), improves yields, and increases fidelity 2-fold compared to Taq DNA polymerase. In addition, the Hot Start technology allows complete reactivation of the enzyme in “zero-time” at standard cycling temperatures. This combination of features makes the polymerase an ideal solution for routine and high throughput PCR applications. Phire® Hot Start II DNA Polymerase delivers superior performance in conventional thermal cyclers as well as in fast instruments, such as the Piko® Thermal Cycler.
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Direct PCR kits
Supplier: FINNZYMES REAGENTS
Direct PCR Kits save time and cost by allowing amplification of DNA directly from the source material. Direct PCR is based on Thermo Scientific Phire® Hot Start II DNA Polymerase and Phusion® Hot Start II High-Fidelity DNA Polymerase. These fusion enzymes are exceptionally fast, robust and highly tolerant of many PCR inhibitors present in unpurified sample material. This allows reliable amplification in challenging conditions where conventional DNA polymerases are completely inhibited.
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PCR master mix (2X)
Supplier: Thermo Fisher Scientific
PCR Master Mix is a 2X concentrated solution of Taq DNA polymerase, dNTPs and all other components required for PCR, except DNA template and primers. This pre-mixed formulation saves time and reduces contamination due to a reduced number of pipetting steps required for PCR set up. The mix is optimised for efficient and reproducible PCR.