Hait: Nucleic Acid Reagents

Supplier: Cytiva

Sera-Xtracta virus/pathogen kit for high-throughput total nucleic acid (DNA/RNA) isolation from bacteria and viruses including adenovirus (type 14), influenza A (H3N2) and COVID-19.

Supplier: EKSIGENT

The DNA 20 kb plasmid and linear kit is used to do isoform and purity analysis of plasmid and linearised dsDNA.

Supplier: BIOPREMIER

The RNA viral extraction kit is designed for the rapid simultaneous purification of viral RNA from cell–free samples, solid samples, swabs and transport media.

Supplier: MP Biomedicals

Humic acids, heavy metals, heme are the most notorious PCR inhibitors. This could be due to non-optimised DNA purification procedures which often co-purify inhibitors and lead to false negative results on downstream applications.

Supplier: Cytiva

Sephadex™ G-50 M DNA Grade chromatography resin for purification of DNA fragments up to 20 bases in length from small molecules such as salts by size exclusion.

Supplier: MP Biomedicals

SPINeasy Plasmid Midiprep kit is a silica-membrane spin-column kit for the purification of high-copy and low-copy number plasmid DNA from 25 to 50 ml of transformed bacterial (E.coli) culture.

Supplier: Corning

The AxyPrep™ Mag Tissue gDNA Kit utilises a magnetic bead-based technology to isolate genomic DNA from rodent tail tissues. DNA from other tissue types may be extracted with this protocol, but optimisation may be required.

Supplier: Cytiva

Sera-Xtracta genomic DNA kit offers magnetic bead-based extraction and purification of genomic DNA from whole blood.

Supplier: MP Biomedicals

SPINeasy PCR purification and gel extraction Kit is a silica-membrane spin-column kit that enables quick and convenient DNA clean up from various enzymatic reactions, such as PCR and restriction digestion, as well as isolation and purification of DNA fragments from agarose gel electrophoresis.

Supplier: Cytiva

The ExoProStar™ S PCR and Sequence Reaction Clean-Up Kit updates existing enzymatic PCR clean up technology with Exonuclease I and Shrimp Alkaline Phosphatase to remove unincorporated primers and dNTPs. illustra ExoProStar™ S improves digestion efficiency with no degradation of the target PCR product. Sample remain completely intact and ready for immediate use in manual or automated processes. For maximum flexibility the enzymes are supplied in two separate tubes.

Supplier: Merck Millipore (Novagen)

The Mouse Ig-Primer Set is designed for amplification of immunoglobulin (Ig) variable region cDNAs. Mammalian light- and heavy-chain Igs contain conserved regions adjacent to the hypervariable complementarity defining regions (CDRs). Appropriately designed oligonucleotide primer sets enable these regions to be specifically amplified using PCR, after which the products may be sequenced directly or cloned.

Supplier: Cytiva

SeraSil-Mag™ silica coated superparamagnetic beads for nucleic acid isolation deliver high purity DNA extraction in NGS applications. These beads provide an optimal surface for nucleic acid binding with high performance and low background.

Supplier: Cytiva

illustra™ MicroSpin™ S-400 HR columns designed for rapid purification of PCR products (>200 bp) from unincorporated primers (<32-mers) and nucleotides using spin-column chromatography.

Supplier: Genscript

The AmMag Quatro is an automated solution for purification of large-scale, high quality, transfection-grade plasmids from Escherichia coli cultures.

Supplier: MP Biomedicals

The SPINeasy® DNA Pro Kit for Feces is able to handle a wide range of fecal samples.

Supplier: MP Biomedicals

Quick and convenient purification of total RNA from animal tissues, plant tissues and tissue cultures using spin column technology.

Supplier: MP Biomedicals

Soil samples are complex environments characterised by the presence of inhibitory compounds, such as humic acid, heavy metals, and other aromatic components which may prove to be challenging for downstream analyses.

Supplier: MP Biomedicals

SPINeasy DNA Kit for plant is a silica-membrane spin-column kit that enables quick, convenient and cost-effective isolation of genomic DNA (gDNA) from various plant tissues, such as leaf, stem and seed.

Supplier: MP Biomedicals

MagBeads FastRNA Kit for FFPE is intended for rapid extraction of RNA from tissue, cells, blood, and other clinical samples. RNA can be used directly for RT-PCR, quantitative RT-PCR and so on.

Supplier: MP Biomedicals

The GeneClean® Turbo for PCR Kit removes primers, nucleotides, enzymes, mineral oil, salts, and other impurities from DNA samples. DNA fragments purified with this kit are ready for direct use in all applications, including sequencing, microarray analysis, ligation and transformation, restriction digestion and labeling.

Supplier: Cytiva

The illustra GFX PCR DNA and Gel Band Purification kits are for the isolation and concentration of DNA fragments from PCR mixtures, DNA-containing agarose gel bands, enzyme-based DNA modifications, and restriction enzyme digests.

Supplier: PRIMERDESIGN

The genesig easy DNA/RNA extraction protocol begins with a simple lysis step where cells and tissue are lysed to release their nucleic acid. Then minute magnetic particles are added to bind to DNA/RNA. When placed on to the genesig magnetic separator the particles are pulled to the side of the tube making it easy to remove the unwanted supernatant with a pipette. Then a series of simple wash steps are performed before the DNA/RNA is washed off the beads back in to solution, ready for analysis by real-time PCR.

It is fast, and incredibly easy to perform.

Supplier: Merck Millipore (Novagen)

The One Step RT-PCR Master Mix Kit allows rapid, sensitive analysis of gene expression from tissues and cells. It can replace methods for detecting and quantifying gene expression such as Northern blots, in situ hybridisation, dot blots, S nuclease assays and conventional two step RT-PCR. The kit utilises recombinant Thermus thermophilus (rTth) DNA Polymerase, which acts as both a thermostable RNA dependent DNA polymerase and a DNA dependent DNA polymerase. The rTth DNA Polymerase is provided in a 2X master mix with an antibody for antibody-mediated hot start, optimised buffer, and ultrapure deoxynucleotides. Antibody-mediated Hot Start enhances specificity of both reverse transcription and PCR. The kit enables cDNA synthesis from input RNA followed by PCR amplification of the cDNA in a single reaction, with no additional hands-on requirement for buffer changes or adding reagents. Typically, detection of a specific transcript requires only 2 hours.

Supplier: Cytiva

illustra™ dNTPs are high purity solutions for enzymatically sensitive applications, such as nucleic acid amplification, sequencing, labelling, mutagenesis, cDNA synthesis and expression profiling.

Supplier: Cytiva

PCR nucleotide mix dNTP set (2 mM, 10 mM, or 25 mM each A,C,G,T) for amplification, dideoxy sequencing, labelling, mutagenesis, cDNA synthesis, and expression profiling.

Supplier: MP Biomedicals

Set consists of 100 mM aqueous solutions of dATP, dCTP, dGTP and dTTP each supplied in a separate vial.

Supplier: Cytiva

Easy to use, time saving solutions for in vitro transcription or for reactions requiring rNTPs of high purity.

Supplier: MP Biomedicals

This dNTP mix with dATP, dCTP, dGTP, dTTP is suitable for use in PCR, real-time PCR, high fidelity and long PCR, LAMP-PCR, cDNA synthesis, RT-PCR, DNA sequencing and labeling.

Supplier: Cytiva

High purity dNTPs for amplification, dideoxy sequencing, labelling, mutagenesis, cDNA synthesis, and expression profiling.

Supplier: MP Biomedicals

Hybrisol VII