Accurately quantifying total protein concentration is a key to most experiments and is often necessary for isolation, separation, and analysis of proteins by biochemical methods. When it is necessary to determine the total protein concentration in a sample, one of the first factors to consider is the selection of a protein assay method. These choices are typically based upon the compatibility of the method with the samples to be assayed. Each method has its own advantages and disadvantages.
Pierce™ GlycoProtein Carbohydrate Estimation Kits
Supplier: Thermo Fisher Scientific
Pierce™ Glycoprotein Carbohydrate Estimation Kit enables the amount of protein glycosylation to be measured as a percentage of total purified protein mass.
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Protein Assay, Protn-Latex™
Supplier: G-Biosciences
A simple and reliable method for estimation of protein contamination in latex. Detects as low as 25 to 40 μg protein/gm latex (or 25 to 50 ppm). Protein solutions applied to the test strips form compact and symmetrical spots, their diameter being directly proportional to protein concentration. Protn-Latex™ latex protein extraction buffer extracts protein from the latex sample, which is then applied to the test strips and rapidly developed. By measuring the Ø of protein spots with the Protn-Latex™ card, the range of protein concentration in the sample can be determined.
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Allergen protein rapid test kits
Supplier: 3M Food Safety
The 3M™ Allergen Protein Rapid Kits are intended for screening for the presence of specific proteins in clean-in-place (CIP) final rinse water, environmental swab samples, food ingredients and processed food products.
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Horseradish Peroxidase, Biotinylated, Pierce™
Supplier: Thermo Fisher Scientific
Biotinylated horse radish peroxidase is used as control or signal amplification in IHC to amplify the signal of biotinylated primary antibodies using the avidin-biotin complex staining method.
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Pierce™ Biotin Quantitation Kit
Supplier: Thermo Fisher Scientific
Pierce™ Biotin Quantitation Kit includes aliquots of HABA reagent and biotinylated protein standard for convenient colorimetric determination of biotinylation levels in labeled antibodies and other proteins.
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Marfey's reagent (FDAA or 1-fluoro-2-4-dinitrophenyl-5-L-alanine amide), Pierce™
Supplier: Thermo Fisher Scientific
Marfey's Reagent (FDAA or 1-fluoro-2-4-dinitrophenyl-5-L-alanine amide) reacts with primary amines to enable the quick and easy separation and quantitation of optical isomers of amino acids by reverse-phase chromatography.
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Biotinylated-protein interaction pull down kit, Pierce™
Supplier: Thermo Fisher Scientific
Pierce™ biotinylated protein interaction pull-down kit contains the necessary components to capture and purify interactors of a biotin-labeled protein or ligand.
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Pierce™ Polyhis-Protein Interaction Pull Down Kit
Supplier: Thermo Fisher Scientific
The Pierce™ his protein interaction pull-down kit contains the necessary components to capture and purify proteins that interact with his-tagged fusion proteins.
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GST protein interaction pull down kit, Pierce™
Supplier: Thermo Fisher Scientific
The Pierce™ GST Interaction Pull-Down Kit contains the necessary components to capture and purify proteins that interact with GST-tagged fusion proteins.
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Accessories for fluorescent protease assay, Fluoro™
Supplier: G-Biosciences
Protein assay, Resorufin-Casein protease substrate
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Accessories for protein assays
Supplier: G-Biosciences
Protein assay, dotMETRIC™ spot application device
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Glycogen Assay Kit
Supplier: Cayman Chemical
A simple and sensitive tool for assaying glycogen from tissue.
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Osteoclast inhibitory lectin MAB MO XMO
Supplier: US Biological
Osteocrin is a recently identified secreted protein expression of which was only detected in bone, peaking just after birth and decreasing markedly with age. A 1280-bp mRNA encodes osteocrin producing a mature protein of 103 amino acids with a molecular mass of 11,4 kD. In primary osteoblastic cell cultures osteocrin expression coincided with matrix formation then decreased in very mature cultures.
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Solid phase biotinylation kits, EZ-Link™, Pierce™
Supplier: Thermo Fisher Scientific
This antibody labeling system uses nickel chelated agarose to temporarily immobilise antibody molecules via their histidine-rich Fc regions. Once held in place on the gel, the antibody can be biotinylated at either sulfhydryl groups (after mild reduction or disulfide bonds) or primary amines. Excess labeling reagent and by-products are then washed away before recovering the labeled and purified antibody from the gel using a mild imidazole solution. No gel filtration or dialysis are needed.
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Quantitative colorimetric peptide assay, Pierce™
Supplier: Thermo Fisher Scientific
Thermo Scientific™ Pierce™ Quantitative Colorimetric Peptide Assay is an easy-to-use colorimetric microplate assay designed specifically to improve the sensitivity and reproducibility of quantitation of peptide mixtures.
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L-Lactate Assay Kit
Supplier: Cayman Chemical
A fluorescence-based method for detecting L-lactate in biological samples such as serum, plasma, blood, urine, and saliva.
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β-Hydroxybutyrate (Ketone Body) Assay Kit
Supplier: Cayman Chemical
A sensitive tool for measuring β-HB levels in plasma, serum, or urine.
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Quantitative fluorometric peptide assay, Pierce™
Supplier: Thermo Fisher Scientific
Thermo Scientific™ Pierce™ Quantitative Fluorescent Peptide Assay is a sensitive, mix-and-read fluorescent microplate assay for the quantitative measurement of peptides and peptide mixtures.
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Foxp3 / Transcription Factor Staining Buffer Kit, Tonbo Biosciences
Supplier: Tonbo Biosciences
Tonbo Biosciences Foxp3 / Transcription Factor Staining Buffer Kit contains specially formulated buffers and solutions for optimal resolution and low background in your analysis of nuclear antigens by flow cytometry
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Streptavidin poly-HRP conjugate, Pierce™
Supplier: Thermo Fisher Scientific
Pierce™ Streptavidin Poly-HRP conjugate is biotin-binding protein conjugated with polymers of horseradish peroxidase, enabling signal amplification and detection of biotinylated antibodies for IHC and other methods.
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Firefly luciferase glow assay kit, Pierce™
Supplier: Thermo Fisher Scientific
The Pierce™ Firefly Luciferase Glow Assay Kit provides a bright and stable bioluminescence signal (half-life greater than 2 hours) in the presence of firefly luciferase reporters for protein expression assays.
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Portelite™ Fluorimetric Protein Quantitation Kit
Supplier: AAT Bioquest
Optimised for CytoCite™ and Qubit™ fluorometers. The Portelite™ fluorimetric protein quantitation kit is significantly more sensitive than existing colorimetric protein measurements, e.g., Bradford and Bicinchoninic acid (BCA) assays.
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Portelite™ Fluorimetric Protein Quantitation Kit, Optimized for CytoCite™ and Qubit™ Fluorometers
Supplier: AAT Bioquest
Protein quantification is an essential task in protein purification, electrophoresis, cell biology, molecular biology and other research applications.
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Tetracysteine REASH Reagent *2 mM DMSO Solution*
Supplier: AAT Bioquest
REASH is a resorufin derivative, modified to contain two arsenic atoms at a set distance from each other.
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Lumiwox™ Acridinium NHS Ester
Supplier: AAT Bioquest
The acridinium NHS ester can be used to label proteins and nucleic acids.
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Octet® Anti-penta-HIS (HIS1K) biosensors
Supplier: Sartorius
For label-free quantitation and kinetic analysis of his-tagged proteins.
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ReadiUse™ TCA Deproteinization Sample Preparation Kit
Supplier: AAT Bioquest
The analysis of amino acids, ions, metabolites and other small molecules is frequently hindered by the interference of lipids, protein and enzymes present in biofluids, cell and tissue lysates.
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Tetracysteine FLASH Reagent *2 mM DMSO Solution*
Supplier: AAT Bioquest
FLASH is a fluorescein derivative, modified to contain two arsenic atoms at a set distance from each other.
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ReadiUse™ TCEP Removal Solution
Supplier: AAT Bioquest
TCEP is a common reducing reagent widely used to break disulfide bonds within and between proteins.
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Surface protein plus test swabs, PRO100, Clean-Trace™
Supplier: 3M Food Safety
3M™ Clean-Trace™ Surface Protein Plus Test Swab PRO100 is a simple, quick, and reliable method of checking for the removal of protein residues after cleaning and quickly identifies contamination.