You searched for: Enzyme Assays

Either measuring substrate consumption or overall production, the enzyme assays are used to monitor enzymatic activity. Provided with the required supplies and reagents for any preferred method, enzyme kinetics and inhibition studies can be successfully completed. While continuous assay sampling methods give activity readings throughout development, discontinuous techniques calculate after the reaction has been stopped. The standardized enzyme assays will provide accurate identification and measurement of the protein molecules present in samples.

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Amplite® Colorimetric Bradford Protein Quantitation Assay Kit

Supplier: AAT Bioquest

The traditional Bradford protein assay is widely used for quantifying protein concentrations.

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Amplite® Fluorimetric L-Alanine Assay Kit

Supplier: AAT BIOQUEST

L-alanine (L-Ala) plays a crucial role as a building block of important proteins.

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Amplite® Fluorimetric Beta-Galactosidase Assay Kit Green Fluorescence

Supplier: AAT BIOQUEST

E. coli beta-galactosidase is a 464 Kd tetramer.

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Amplite® Universal Fluorimetric Protease Activity Assay Kit Green Fluorescence

Supplier: AAT BIOQUEST

Monitoring of various protease activities has become a routine task for many biological laboratories.

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Amplite® Fluorimetric Butyrylcholinesterase Activity Assay Kit

Supplier: AAT BIOQUEST

Butyrylcholinesterase (EC 3.1.1.8; BChE), also known as pseudocholinesterase or plasma cholinesterase, is mainly synthesized liver and present in blood.

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Amplite® Colorimetric NADP/NADPH Ratio Assay Kit

Supplier: AAT BIOQUEST

Nicotinamide adenine dinucleotide (NAD+) and nicotinamide adenine dinucleotide phosphate (NADP+) are two important cofactors found in cells.

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Amplite® Fluorimetric Total NAD and NADH Assay Kit Red Fluorescence

Supplier: AAT BIOQUEST

Nicotinamide adenine dinucleotide (NAD+) and nicotinamide adenine dinucleotide phosphate (NADP+) are two important cofactors found in cells.

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Amplite® Fluorimetric Hydrogen Peroxide Assay Kit Red Fluorescence

Supplier: AAT BIOQUEST

Hydrogen peroxide (H₂O₂) is a reactive oxygen metabolic by-product that serves as a key regulator for a number of oxidative stress-related states.

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Amplite® Fluorimetric Neutrophil Elastase Activity Assay Kit

Supplier: AAT BIOQUEST

Designed for consistent and dependable results. The Amplite® fluorimetric neutrophil elastase activity assay kit provides a robust and accurate method for quantifying neutrophil elastase (NE) activity.

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Amplite® Colorimetric Ammonia Quantitation Kit Blue Color

Supplier: AAT BIOQUEST

Ammonia is an important source of nitrogen for living systems.

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Amplite® Colorimetric Urea Quantitation Kit Blue Color

Supplier: AAT BIOQUEST

Urea is the final degradation product of protein and amino acid metabolism in animals.

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Amplite® Colorimetric Zinc Ion Quantitation Kit

Supplier: AAT BIOQUEST

Zinc is an essential trace mineral element that plays an important role in a number of biological processes.

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Amplite® Colorimetric α-Ketoglutarate Quantitation Kit

Supplier: AAT BIOQUEST

Alpha-ketoglutarate (α-Ketoglutarate) is a key molecule in the Krebs cycle determining the overall rate of the citric acid cycle of the organism.

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Amplite® Fluorimetric Zinc Ion Quantitation Kit

Supplier: AAT BIOQUEST

Zinc is an essential trace mineral element that plays an important role in a number of biological processes.

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Amplite® Colorimetric Glucose and Sucrose Assay Kit

Supplier: AAT BIOQUEST

Designed for consistent and dependable results. The Amplite® Colorimetric Glucose and Sucrose Assay Kit offers a convenient method for measuring glucose and sucrose levels in various biological samples, such as serum, plasma, body fluids, food, tissue extracts, and growth medium.

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Amplite® Fluorimetric Total Thiol Quantitation Assay Kit Green Fluorescence

Supplier: AAT BIOQUEST

The detection and measurement of free thiol (such as free cysteine, glutathione and cysteine residues in proteins) is one of the essential tasks for investigating biological processes and events in many biological systems.

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Amplite® Rapid Fluorimetric Total Thiol Quantitation Assay Kit Green Fluorescence

Supplier: AAT BIOQUEST

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Amplite® Fluorimetric Coenzyme A Quantitation Kit Green Fluorescence

Supplier: AAT BIOQUEST

Coenzyme A (CoA) is a universal and essential cofactor in all forms of cellular life acting as a principal acyl carrier in numerous biosynthetic, energy-yielding, and degradative pathways.

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β-Galactosidase Assay Kit and Reagents

Supplier: Thermo Fisher Scientific

The Mammalian β-Galactosidase Assay Reagent Kit is a simple and versatile assay to measure β-galactosidase activity in cultured mammalian cells. Cells grown on a plate may be lysed and tested for β-galactosidase activity by adding a single all-in-one lysis-and-assay solution. Alternatively, M-PER™ Mammalian Protein Extraction Reagent may be used to prepare lysate from washed adherent or pelleted cells, and then a portion of the lysate used to assay for β-galactosidase activity. With M-PER Reagent, there is no need to scrape cells or to perform freeze-thaw cycles. The remaining lysates prepared with M-PER™ Reagent may be used for applications such as reporter assays (luciferase, β-galactosidase and chloramphenical acetyltransferase), protein kinase assays (PKA, PKC, and tyrosine kinase), immunoassays (Western blot, ELISA, RIA) and protein purification.

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Amplite® Fluorimetric L-Lactate Dehydrogenase (LDH) Assay Kit

Supplier: AAT BIOQUEST

Lactate dehydrogenase (LDH) is an oxidoreductase enzyme that catalyses the interconversion of pyruvate and lactate.

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Amplite® Fluorimetric Alkaline Phosphatase Assay Kit Blue Fluorescence

Supplier: AAT BIOQUEST

Alkaline phosphatase is a highly sensitive enzyme for ELISA, immuno-histochemical, Northern, Southern and Western blot applications.

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Amplite® Fluorimetric Glycerol Assay Kit

Supplier: AAT BIOQUEST

Glycerol is a precursor for the synthesis of triglycerides and phospholipids in liver and adipose tissue.

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Amplite® Fluorimetric Glucose-6-Phosphate Assay Kit

Supplier: AAT BIOQUEST

Glucose-6-phosphate (G6P) is a key intermediate for glucose transport into cells.

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Amplite® Renilla Luciferase Reporter Gene Assay Kit Bright Glow

Supplier: AAT BIOQUEST

Common reporter genes include beta-galactosidase, beta-glucuronidase and luciferase.

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Amplite® Gaussia Luciferase Reporter Gene Assay Kit Bright Glow

Supplier: AAT BIOQUEST

Common reporter genes include beta-galactosidase, beta-glucuronidase and luciferase.

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Amplite® Fluorimetric Lipase Activity Assay Kit

Supplier: AAT BIOQUEST

Designed for consistent and dependable results. The Amplite® Fluorimetric Lipase Activity Assay Kit provides a simple and quick protocol for measuring lipase activity.