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Either measuring substrate consumption or overall production, the enzyme assays are used to monitor enzymatic activity. Provided with the required supplies and reagents for any preferred method, enzyme kinetics and inhibition studies can be successfully completed. While continuous assay sampling methods give activity readings throughout development, discontinuous techniques calculate after the reaction has been stopped. The standardized enzyme assays will provide accurate identification and measurement of the protein molecules present in samples.

Amplite® Colorimetric L-Aspartate (Aspartic Acid)  Assay Kit

Amplite® Colorimetric L-Aspartate (Aspartic Acid) Assay Kit

Supplier: AAT BIOQUEST

Aspartate (or Aspartic acid) is a negatively charged, polar amino acid.

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Amplite® Fluorimetric D-Lactate Assay Kit

Amplite® Fluorimetric D-Lactate Assay Kit

Supplier: AAT BIOQUEST

Lactic acid is chiral and has two optical isomers: L-lactic acid and D-lactic acid.

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Amplite® Fluorimetric Myeloperoxidase Assay Kit *Red Fluorescence*

Amplite® Fluorimetric Myeloperoxidase Assay Kit *Red Fluorescence*

Supplier: AAT BIOQUEST

Myeloperoxidase (MPO), most abundantly present in neutrophils and monocytes, is a green hemoprotein having peroxidase activity.

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Amplite® Luciferase Reporter Gene Assay Kit *Bright Glow*

Amplite® Luciferase Reporter Gene Assay Kit *Bright Glow*

Supplier: AAT BIOQUEST

Common reporter genes include beta-galactosidase, beta-glucuronidase and luciferase.

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Amplite® Fluorimetric Monoamine Oxidase Assay Kit *Red Fluorescence*

Amplite® Fluorimetric Monoamine Oxidase Assay Kit *Red Fluorescence*

Supplier: AAT BIOQUEST

Monoamine oxidases (MAO) are a family of flavin-containing amine oxidoreductases that catalyse the oxidation of monoamines.

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Amplite® Colorimetric Peroxidase (HRP) Assay Kit *Blue Color*

Amplite® Colorimetric Peroxidase (HRP) Assay Kit *Blue Color*

Supplier: AAT BIOQUEST

Peroxidase is a small molecule (MW ~40 KD) that can usually be conjugated to an antibody in a 4:1 ratio.

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Amplite® Fluorimetric Sphingomyelinase Assay Kit *Red Fluorescence*

Amplite® Fluorimetric Sphingomyelinase Assay Kit *Red Fluorescence*

Supplier: AAT BIOQUEST

Five types of sphingomyelinase (SMase) have been identified based on their cation dependence and pH optima of action.

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Amplite® Fluorimetric NADH Assay Kit *Red Fluorescence*

Amplite® Fluorimetric NADH Assay Kit *Red Fluorescence*

Supplier: AAT BIOQUEST

Nicotinamide adenine dinucleotide (NAD+) and nicotinamide adenine dinucleotide phosphate (NADP+) are two important cofactors found in cells.

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MMP-3 Green™ Substrate

MMP-3 Green™ Substrate

Supplier: AAT BIOQUEST

The matrix metalloproteinases (MMPs) constitute a family of zinc-dependent endopeptidases that function within the extracellular matrix.

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Pierce™ Gaussia Luciferase Flash Assay Kit

Pierce™ Gaussia Luciferase Flash Assay Kit

Supplier: Thermo Fisher Scientific

The Thermo Scientific Pierce Gaussia Luciferase Flash Assay Kit provides researchers with the most sensitive assay for transcriptional activity of regulatory elements in mammalian cell culture media and whole cell lysate.

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Amplite® Colorimetric Asparaginase Activity Assay Kit

Supplier: AAT BIOQUEST

Designed for consistent and dependable results. The Amplite® colorimetric asparaginase activity assay kit provides a simple and straightforward procedure for measuring asparaginase activity in a variety of biological samples.

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Amplite® Fluorimetric DPP4 Activity Assay Kit

Supplier: AAT BIOQUEST

Designed for consistent and dependable results. The Amplite® fluorimetric DPP4 activity assay kit provides a simple, quick, and direct protocol for measuring DPP4 Activity.

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Amplite® Fluorimetric Aspartate Aminotransferase (AST) Assay Kit

Amplite® Fluorimetric Aspartate Aminotransferase (AST) Assay Kit

Supplier: AAT BIOQUEST

Aspartate aminotransferase (AST), also called serum glutamic oxaloacetic transaminase (GOT), is a member of transferase family.

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MMP Red™ Substrate

MMP Red™ Substrate

Supplier: AAT BIOQUEST

The matrix metalloproteinases (MMPs) constitute a family of zinc-dependent endopeptidases that function within the extracellular matrix.

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Amplite® Fluorimetric Proteasome 20S Activity Assay Kit *Green Fluorescence*

Amplite® Fluorimetric Proteasome 20S Activity Assay Kit *Green Fluorescence*

Supplier: AAT BIOQUEST

Our Amplite® Fluorometric 20S Proteasome Assay Kit is a homogeneous fluorescent assay that measures the chymotrypsin-like protease activity associated with the proteasome complex either in cultured cells or cell lysates.

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Amplite® Fluorimetric Beta-Hydroxybutyrate (Ketone Body) Assay Kit

Amplite® Fluorimetric Beta-Hydroxybutyrate (Ketone Body) Assay Kit

Supplier: AAT BIOQUEST

Ketone bodies are produced by the liver and used peripherally as an energy source when blood glucose levels drop.

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Amplite® Colorimetric Glucose Oxidase Assay Kit

Amplite® Colorimetric Glucose Oxidase Assay Kit

Supplier: AAT BIOQUEST

The glucose oxidase is a dimeric protein that catalyses the oxidation of beta-D-glucose into hydrogen peroxide and D-glucono-1,5-lactone, which is hydrolysed to gluconic acid.

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Amplite® Fluorimetric Glucose-6-Phosphate Dehydrogenase (G6PD) Assay Kit

Amplite® Fluorimetric Glucose-6-Phosphate Dehydrogenase (G6PD) Assay Kit

Supplier: AAT BIOQUEST

Glucose-6-phosphate dehydrogenase (G6PD) catalyses the conversion of glucose-6-phosphate to 6-phosphoglucono-δ-lactone, the first and rate-limiting step in the pentose phosphate pathway.

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Amplite® Colorimetric Gama-Glutamyltransferase (GGT) Activity Assay Kit

Supplier: AAT BIOQUEST

Designed for consistent and dependable results. The Amplite® Colorimetric Gamma-Glutamyltransferase (GGT) Activity Assay Kit provides an efficient and straightforward method for measuring GGT activity across various sample types.

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Amplite® Red Peroxidase Substrate

Amplite® Red Peroxidase Substrate

Supplier: AAT BIOQUEST

Amplite® Red is a sensitive fluorogenic peroxidase substrate that generates a highly red fluorescent product that has maximum absorption of 571 nm and maximum emission of 585 nm.

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Pierce™ Cypridina Luciferase Glow Assay Kit

Pierce™ Cypridina Luciferase Glow Assay Kit

Supplier: Thermo Fisher Scientific

The Thermo Scientific Pierce Cypridina Luciferase Glow Assay Kit provides an extremely bright and stable bioluminescence signal (half-life greater than 1 hour) in the presence of Cypridina luciferase reporters.

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Amplite® Colorimetric Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) Assay Kit

Supplier: AAT BIOQUEST

Designed for consistent and dependable results. The Amplite® colorimetric GAPDH activity assay kit is a sensitive and simple tool for monitoring GAPDH activity based on a coupled enzymatic reaction catalysed by GAPDH.

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Amplite® Rapid Fluorimetric Glutathione GSH/GSSG Ratio Assay Kit *Green Fluorescence*

Amplite® Rapid Fluorimetric Glutathione GSH/GSSG Ratio Assay Kit *Green Fluorescence*

Supplier: AAT BIOQUEST

When cells are exposed to increased levels of oxidative stress, GSSG will accumulate and the ratio of GSH to GSSG will decrease.

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Amplite® Fluorimetric Peroxynitrite Quantification Kit *Green Fluorescence*

Amplite® Fluorimetric Peroxynitrite Quantification Kit *Green Fluorescence*

Supplier: AAT BIOQUEST

Peroxynitrite (ONOO-) is a strong oxidizing species and a highly active nitrating agent.

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Amplite® Fluorimetric Lead (II) Ion Quantitation Kit

Amplite® Fluorimetric Lead (II) Ion Quantitation Kit

Supplier: AAT BIOQUEST

Lead is a highly poisonous metal to human beings, especially young children.

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Gaussia luciferase glow assay kit, Pierce™

Gaussia luciferase glow assay kit, Pierce™

Supplier: Thermo Fisher Scientific

The Thermo Scientific Pierce Gaussia Luciferase Glow Assay kit provides an extremely bright and stable bioluminescence signal (half-life greater than one hour), especially in the presence of Gaussia-Dura luciferase reporter.

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Amplite® Fluorimetric Endotoxin Detection Kit

Amplite® Fluorimetric Endotoxin Detection Kit

Supplier: AAT BIOQUEST

Lipopolysaccharide (LPS), also known as endotoxin, is the major component of the outer membrane of Gram-negative bacteria.

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Amplite® Ethanol Quantitation Kit

Amplite® Ethanol Quantitation Kit

Supplier: AAT BIOQUEST

The ability to rapidly perform quantitative measurements of ethanol is highly desirable in life science research, clinical evaluations, and food and drug industries.

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Amplite® Fluorimetric α-Ketoglutarate Quantitation Kit

Amplite® Fluorimetric α-Ketoglutarate Quantitation Kit

Supplier: AAT BIOQUEST

Alpha-ketoglutarate (α-ketoglutarate) is a key molecule in the Krebs cycle determining the overall rate of the citric acid cycle of the organism.

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Peroxide assay kit, PEROXsay™

Peroxide assay kit, PEROXsay™

Supplier: G-Biosciences

PEROXsay™ is a colorimetric quantitative peroxide assay that measures the oxidation of ferrous (Fe²⁺+) ions to ferric (Fe³⁺) ions. Peroxides react with a sugar alcohol converting it to a peroxyl radical that subsequently starts the oxidation of ferrous ions to ferric ions. The acidic pH of the assay allows the ferric (Fe³⁺) ion to complex with the colorimetric reagent, resulting in a change in absorbance that is proportional to the peroxide concentration.

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