Overcome normal optical limitations in microscopic studies by highlighting specific structures with stains and reagents and then protect samples with fixatives. The fast-acting dyes create strong contrasts between cell components for easier detection or analyses. Sensitive attractions to certain organelle or cellular components differ among offered stains, allowing precise testing modifications. Flexible in use, ready-to-use stains may be handled manually or in conjunction with automatic equipment.
VWR® VividPro™ Fluorescent Stains
Supplier: VWR Chemicals
VividPro™ is a generic fluorescent stain for protein gel electrophoresis that can be used to visualize proteins.
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VWR® VividPro™ Plus Fluorescent Protein Stains
Supplier: VWR Chemicals
VividPro Plus™ is a fluorescent protein stain that can be used in a fast co-electrophoretic protocol to visualize proteins in SDS PAGE gels.
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Differentiation Solution
Supplier: SIGMA ALDRICH MICROSCOPY
Differentiation solution comprises acidified ethanol or isopropanol that is used to decolourise hematoxylin stain. The duration of exposure to the solution determines the degree of decolourisation. Differentiation is a crucial step in hematoxylin-eosin staining to achieve the proper colour contrast necessary for visualisation, by selectively removing excess hematoxylin from the tissue section and leaving behind the desired staining intensity.
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VWR® BANDit™ Protein Stain Blue
Supplier: VWR Chemicals
Blue BANDit is a safe and environmentally friendly alternative to traditional Coomassie® Blue staining.
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Aqueous mounting medium, Fluoromount™, Sigma-Aldrich®
Supplier: SIGMA ALDRICH MICROSCOPY
Fluoromount™ Aqueous Mounting Medium for use with fluorescent dye-stained tissue.
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Neutral mounting medium, DPX, Sigma-Aldrich®
Supplier: SIGMA ALDRICH MICROSCOPY
D.P.X mounting medium is a mixture of distyrene (polystyrene), a plasticizer (tricresyl phosphate), and xylene. It facilitates the protection of stains and can dry quickly.
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Osteo-Bed Bone Embedding Solvent
Supplier: SIGMA ALDRICH MICROSCOPY
For use with Osteo-bed bone embedding kit. Solvent for removal of plastic from sections prior to rehydration and staining. Most histochemical and immunohistochemistry staining protocols require the removal of methyl methacrylate (MMA) and rehydration of mounted sections for improved stain penetration and greater contrast.
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Aqueous mounting medium Aquatex®, Sigma-Aldrich®
Supplier: Merck
Aquatex is used for e.g. LEUCOGNOST stained specimens. For preservation purposes and subsequent optimal microscopy, the stained sections are normally mounted using a suitable mounting agent and covered with a cover glass.
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VWR® EZ-Vision® Bluelight DNA Dye 10000X
Supplier: VWR Chemicals
EZ-Vision BlueLight is a sensitive, non mutagenic and environmentally safe fluorescent dye designed for gel staining.
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VWR® EZ-Vision®, DNA dyes
Supplier: VWR Chemicals
EZ-Vision DNA dye as loading buffer provides a non mutagenic, environmentally friendly alternative to ethidium bromide for instantaneous fluorescent DNA band visualisation.
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VWR®, DNA loading buffers
Supplier: VWR Chemicals
These DNA loading dyes are used to load DNA samples to agarose or SDS DNA gels for gel electrophoresis.
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VWR® Gold-N-Gel™, RNA dye
Supplier: VWR Chemicals
Gold-N-Gel RNA dye is compatible with standard protocols for denaturing electrophoresis of RNA fragments. Bands are immediately visible upon illumination with UV light. A standard ethidium bromide filter can be used for gel documentation.
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M-FIX™ Spray fixative for gynecological and clinical cytology, Sigma-Aldrich®
Supplier: Merck
M-FIX is a fixative spray designed for the wet fixation of gynaecological and non-gynaecological preparations processed in Papanicolaou staining. It contains polyglycols in alcoholic solution and a rapid and secure fixation of specimens is possible.
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Zinc Formalin Fixative
Supplier: SIGMA ALDRICH MICROSCOPY
Zinc formalin fixative is a non-precipitating fixative. This fixative is compatible with histochemical, immunochemical and special stains. It may be used with automated tissue processors and also in manual methods. The zinc chloride component of the formulation increases the rigidity of cellular components to withstand subsequent processing, sectioning and staining procedures. Tissue specimens initially fixed using 10% neutral buffered formalin solution may be post-fixed using the zinc formalin fixative.
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VWR®, Loading dye base
Supplier: VWR Chemicals
This loading dye base is used for custom loading buffer preparation. It is a concentrated solution containing patent blue, bromophenol blue and amaranth tracking dyes, which migrate at approximately 4 000, 400 and 10 bp, on a 1% agarose gel, respectively.
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Electrophoresis gel stain, Reversible Zinc Stain™
Supplier: G-Biosciences
A single step stain for the rapid detection of proteins resolved by PAGE. The stain is based on the interaction of zinc ions with polyacrylamide and proteins.
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Mountant for histology, DPX, Sigma-Aldrich®
Supplier: SIGMA ALDRICH MICROSCOPY
DPX Mountant for histology has been used as a slide mountant to fix sections.
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Sigma-Aldrich® Bouin's solution
Supplier: SIGMA ALDRICH MICROSCOPY
Bouin's solution is an excellent fixative for preserving soft and delicate structures. It is prepared with saturated picric acid, formaldehyde and acetic acid.
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Electrophoresis gel stain, Reversible Copper Stain™
Supplier: G-Biosciences
The Reversible Copper Stain™ is a single step stain for the rapid detection of proteins resolved on SDS-PAGE.
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Immersion oil for microscopy, Sigma-Aldrich®
Supplier: Merck
Used to increase lens effectiveness
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VWR®, Nucleic acid loading dyes
Supplier: VWR Chemicals
Designed to optimise loading nucleic acids in agarose gels, the loading dye imparts colour to the sample to facilitate the loading process and increase the density of the sample to ensure efficient sample distribution into each well. The dye migrates independently from the sample, allowing estimation of the migration of nucleic acids.
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Aqueous mounting medium, Fluoroshield™ with DAPI, Sigma-Aldrich®
Supplier: SIGMA ALDRICH MICROSCOPY
Fluoroshield™ with DAPI is an aqueous mounting medium for preserving fluorescence of tissue and cell smears.
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RenLam® M-1 for microscopy, suitable as a embedding medium, Sigma-Aldrich®
Supplier: SIGMA ALDRICH MICROSCOPY
To be used with SIAL 69571 Ren HY 956 (Hardener for Epoxy Embedding Medium)
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ImmunoHistoMount™ Mounting Medium
Supplier: SIGMA ALDRICH MICROSCOPY
ImmunoHistoMount™ is a permanent aqueous mounting medium designed for tissue sections and cell smears with peroxidase and alkaline phosphatase chromogens that cannot be dehydrated with organic solvents.
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Ren™ HY 956 epoxy embedding medium, hardener, Sigma-Aldrich®
Supplier: SIGMA ALDRICH MICROSCOPY
to be used with SIAL 78711 RenLam M-1 (Epoxy Embedding Medium)
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Immersion oil viscosity 1,250 cSt (lit.), Sigma-Aldrich®
Supplier: SIGMA ALDRICH MICROSCOPY
Immersion oil has been used in epifluorescent microscopy and cell identification.
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Glycoprotein stain
Supplier: G-Biosciences
The stain uses an enhanced Periodic Acid-Schiff (PAS) method for detection of glycoprotein sugars. The supplied agent first oxidises the cis-diol sugar groups to aldehydes. The aldehyde groups react with the sensitive glycoprotein stain solution forming Schiff bonds and producing strong magenta colour bands. RAPIDstain™ can be used after glycoprotein staining to detect non-glycosylated proteins and the use of the stain enhances glycoprotein staining. The glycoprotein stainis highly convenient as all the key reagents required for staining are supplied and a unique positive & negative control is included.
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Immersion oil for microscopy, Sigma-Aldrich®
Supplier: SIGMA ALDRICH MICROSCOPY
Immersion oil is used for high resolution (1000X) light microscopy work under oil immersion objective lens.
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Aqueous mounting medium, Fluoroshield™, Sigma-Aldrich®
Supplier: SIGMA ALDRICH MICROSCOPY
Fluoroshield™ is an aqueous mounting medium for preserving fluorescence of tissue and cell smears.
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HistoChoice® Clearing agent, Sigma-Aldrich®
Supplier: SIGMA ALDRICH MICROSCOPY
HistoChoice® is a patented, complex mixture of agents that allow tissues to retain their structure, antigenic sites and nucleic acid sites. This formulation in non-citrus, contains no mercury, formaldehyde or glutaraldehyde.