You searched for: Protein Linking and Labeling Systems

Supplier: Thermo Fisher Scientific

DyLight™ PEGylated dyes display absorption spectra ranging from 550 nm to 783 nm. These reagents fluoresce over a broad pH range, are more intense than Alexa Fluor® or Cy® dyes in many applications and match the output wavelengths of common fluorescence instrumentation. Additionally, the water solubility of the DyLight™ reagents allows a high dye-to-protein ratio without precipitation during conjugation.

Supplier: Thermo Fisher Scientific

The DyLight™ Specialty Dyes are fluorescent dyes with varying spectral characteristics, degrees of sulfonation, charges and hydrophobicities. These dyes are offered in their amine-reactive forms and are mainly derivatized with N-hydroxysuccinimide (NHS) esters, the simplest and most commonly used reactive group for labeling proteins. The large selection of near infrared (NIR) and infrared (IR) dyes may be used for in vivo and near-IR fluorescence (NIRF) applications.

Supplier: Thermo Fisher Scientific

The DyLight™ Long Stokes Shift Dyes are amine-reactive fluorescent dyes that produce long Stokes (LS) shifts of 80 to 145 nm in excitation/emission wavelengths. The DyLight™ LS dyes provide multicolour detection using a single excitation source in applications including flow cytometry, conjugation, DNA-sequencing, microscopy and fluorescence in situ hybridization (FISH). These dyes contain N-hydroxysuccinimide (NHS) esters, the most commonly used reactive group for labeling proteins. NHS esters react with primary amines on proteins and other molecules, forming a stable, covalent amide bond and releasing the NHS group.

Supplier: G-Biosciences

HOOK™ Biotin-Hydrazide and its long spacer arm equivalent, HOOK™ Biotin-LC-Hydrazide, are carbohydrate reactive reagents. HOOK™ carbohydrate reactive biotin reagents react with oxidised carbohydrate side chains. Some biotin reagents do not bind directly to the protein itself, but instead, conjugate to the carbohydrate residues of glycoproteins. The carbohydrate reactive biotin reagents contain hydrazides (-NH-NH₂) as a reactive group. The hydrazide reactions require carbonyl groups, such as aldehydes and ketones, which are formed by oxidative treatment of the carbohydrates. Hydrazides react spontaneously with carbonyl groups, forming a stable hydrazone bond. These reagents are particularly suitable for labelling and studying glycosylated proteins, such as antibodies and receptors.

Supplier: Thermo Fisher Scientific

The DyLight™ NHS Ester Quenchers are amine-reactive non-fluorescent dyes that use fluorescent resonance energy transfer (FRET) to quench fluorescent signals resulting from ultraviolet, visible and infrared light-excited fluorescent dyes. These dyes can be efficiently paired with many DyLight™ dyes and other common fluorescent dyes including fluorescein, rhodamine, cyanine and Texas Red® dye.

Supplier: G-Biosciences

HOOK™ Biotin-PEG₂-Amine, and its long chain form, HOOK™ Biotin-PEG₃-Amine, are carboxyl reactive biotinylation reagents. These agents contain terminal amines and react with carboxyl groups found at the carboxyl termini, aspartate, and glutamate side chains. The reaction is mediated by a water-soluble carbodiimide (EDC). The carbodiimide activates the carboxyl group and reacts with the amines (-NH₂) on the biotinylation agent to form an amide bond. This reaction is rapid and takes just a few minutes to complete. Under these conditions, hydrazide-derivatives of biotin reagents may also react with the carboxyls.

Supplier: Biotium

Mix-n-Stain™ Digoxigenin antibody labelling kits utilise a technology that allows rapid antibody labelling with digoxigenin without a purification step.

Supplier: Biotium

Biotinylated probes for detection of His-tagged proteins immobilised on nitrocellulose membranes.

Supplier: Merck Millipore (Calbiochem‎)

General proteolytic substrate. Provides a rapid, simple method for the detection of proteases in aqueous solutions.

Supplier: Biotium

Mix-n-Stain™ DNP antibody labelling kits utilise a technology that allows rapid antibody labelling with dinitrophenol without a purification step.

Supplier: Tonbo Biosciences

Ghost Dye Red 710 is an amine reactive viability dye, that can be used to discriminate viable from non-viable mammalian cells in flow cytometry applications.

Supplier: Merck Millipore (Calbiochem‎)

FluorSave™ reagent is an aqueous mounting medium for use in conjunction with fluorescent tracers such as fluorescein, rhodamine and phycobiliprotein probes.

Supplier: Merck Millipore (Oncogene)

Biotin-NHS reacts with primary amines to biotinylate proteins under mild conditions. Since it is water-soluble, this compound can be used to biotinylate cell-surface proteins and proteins that are sensitive to organic solvents.

Supplier: G-Biosciences

The HOOK™ Peptide Coupling kits allow researchers to attach peptides to a carrier protein of choice using either primary amines or sulfhydryls in their peptide. The HOOK™ Peptide Coupling kits can be supplied with or without carrier proteins.

Supplier: Thermo Fisher Scientific

Pierce™ High Sensitivity NeutrAvidin™-HRP Conjugate is a specially prepared peroxidase conjugated form of avidin biotin binding protein that provides signal amplification like poly-HRP and exceptional storage stability.

Supplier: Merck Millipore (Calbiochem‎)

Bilirubin conjugate, ditaurate is suitable as a direct bilirubin standard. It behaves similarly to purified bilirubin glucuronides from human bile in diazo tests.

Supplier: Merck Millipore (Calbiochem‎)

Biotinylated heparin contains 5 − 8 mole-percent biotin. Biotin attachment is through a 12-atom spacer arm containing a cleavable disulfide group.

Supplier: Thermo Fisher Scientific

The Pierce™ Immunostain Enhancer is compatible with fluorescent and chromogenic detection and routinely increases both signal intensity and detection sensitivity.

Supplier: Thermo Fisher Scientific

Cross linkers are used for the identification of near-neighbour protein relationships and ligand-receptor interactions. Homobifunctional, amine-reactive NHS esters or imidates, and heterobifunctional, amine-reactive, photoactivatable phenyl azides are the most commonly used crosslinkers for these applications.

Supplier: Thermo Fisher Scientific

DyLight™ fluor-free acids are non-activated forms of selected DyLight™ fluorescent dyes for use as controls and calibration factors in fluorescence imaging applications. These non-activated fluorescent dyes contain the native carboxyl group of the standard dye molecule.

Supplier: Thermo Fisher Scientific

Maleimide-Activated Streptavidin is for directly preparing streptavidin-protein conjugates with proteins, peptides and other molecules that contain a free sulphhydryl (-SH) group. Streptavidin has been maleimide-activated using Sulpho-SMCC, a heterobifunctional crosslinker that contains an N-hydroxysuccinimide ester and a maleimide group. The activated streptavidin presents an available maleimide group that reacts with sulphhydryl-containing molecules.

Supplier: G-Biosciences

HOOK™ Biotin Reagent that conjugates to proteins through amines, sulfhydryls, carboxyls or carbohydrates. The amine and sulfhydryl coupling HOOK™ Biotin Reagents couple directly to the protein through their reactive groups, however the carboxyl coupling HOOK™ Biotin Reagents require a carbodiimide crosslinker and the carbohydrate coupling HOOK™ Biotin Reagents require carbohydrate oxidation before coupling.

Supplier: Biotium

A far-red passive reference dye for qPCR that frees up the ROX channel for use with JUN™ probes, EvaRuby™ qPCR dye, and other orange dyes.

Supplier: Cytiva

CyDye Fluorescent Dyes are supplied as highly water soluble, reactive NHS esters for easy conjugation to biomolecules containing aliphatic amino groups.

Supplier: Cytiva

Mono-functional maleimides are particularly suitable for the selective labelling of molecules containing free sulfhydryl groups, such as cysteine residues in proteins and peptides and oligonucleotides.

Supplier: Cytiva

The Amersham™ QuickStain Kit contains a Cy™5 fluorophore and labeling buffer for easy detection of proteins in SDSPAGE gels and on Western blotting membranes. The ready to use Cy5 N-hydroxysuccinimide (NHS) ester and Tris labeling buffer ensure robust and consistent labeling for detection of proteins in diverse samples.

Supplier: Cytiva

Hydrazide esters provide a convenient method for the labelling of free carbonyl groups of glycoproteins and carbohydrates. For maximum convenience, stable active hydrazide esters are supplied and stored as solid material.

Supplier: Cytiva

Mono-reactive dye pack contains derivatised CyDye™ that carries only one reactive group on each dye molecule for targeted and specific labelling of amine groups, for example on antibodies, peptides or oligonucleotides.

Supplier: Cytiva

Mono-reactive dye pack contains derivatised CyDye™ that carries only one reactive group on each dye molecule for accurate labelling of amine groups, for example on antibodies or oligonucleotides.

Supplier: Cytiva

Fluorescent nucleotides for labelling DNA and RNA probes, especially during multi-colour analysis in microarrays, FISH, chromosome identification, whole chromosome painting, karyotyping, and gene mapping.