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Buffers

Resisting pH changes, buffers protect samples when acids or alkali is added. Powder and liquid forms efficiently track proteins during electrophoresis isolation and separation. The salty buffers can also be used to calibrate and standardize pH meters for accurate readings. Blocking substances stop antibody attachment to membrane free sites that would produce an interfering background. In various concentrations to fit any need, the buffers may come in liquids or dehydrated formats.

Buffer solutions as per DIN 19266-01: 2000

Buffer solutions as per DIN 19266-01: 2000

Supplier: WTW

These standard buffer solutions (work reference buffer solutions) are widely used in precision applications.

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HEPES protein extraction buffer (5X)

Supplier: Thermo Fisher Scientific

Thermo Fisher Scientific

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Ionisation buffer solutions (CRMs), for AAS and ICP, Fluka™

Supplier: Honeywell Chemicals

Matrix Modifier, 2% Potassium (K) in 1% HCl 1%

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10% SDS (Sodium Dodecyl Sulfate), Corning®

10% SDS (Sodium Dodecyl Sulfate), Corning®

Supplier: Corning

Sodium Dodecyl Sulfate (SDS) is an anionic detergent with applications in SDS-PAGE protein analysis and nucleic acid purification. It is a wetting agent that is effective in both acid and alkaline solutions, and is most often used as a protein and lipid solubilization reagent. SDS is also a powerful protein denaturant. This 10% solution is prepared in 18 megaohm water and is 0.2µm filtered.

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EDTA, 0.5M pH 8.0, Corning®

EDTA, 0.5M pH 8.0, Corning®

Supplier: Corning

EDTA (ethylenediaminetetraacetic acid) is a polyamino carboxylic acid. It is a colorless, water-soluble solid and is useful as a chelating agent due to its ability to "sequester" metal ions such as Ca2+ and Fe3+.  These cations are responsible for various undesirable effects in molecular biological and cell culture processes. After being bound by EDTA, these metal ions remain in the solution but exhibit diminished reactivity.  The use of EDTA therefore allows for greater control over many experiments.

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Buffer solution pH 7, Phosphate buffer

Supplier: Thermo Fisher Scientific

Potassium dihydrogen phosphate and di-Sodium hydrogen phosphate in aqueous solution, Phosphate, pH value: 7,00, ±0,02

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Buffer, sodium acetate, 3 M aq, soln,, pH 7,0, autoclaved

Supplier: Thermo Fisher Scientific

Sodium acetate, 3M aq, solution, pH 7,0, autoclaved

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Phosphate Buffer Mixed pH 4,0

Supplier: CPACHEM

Shelf Life on Ship Date: 12 months - Store under normal laboratory conditions, at temperatures between 15 to 25 °C.

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Buffer, sodium acetate, 1M aq, soln,, pH 4,5, RNAse free

Supplier: Thermo Fisher Scientific

Sodium acetate, 1M aq, solution, pH 4,5, RNAse free, Odourless, Liquid

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Buffer, PBS solution 10X concentrate (phosphate buffered saline)

Supplier: EUROCLONE

Washing buffer, PBS buffer solution 10X concentrate (phosphate buffered saline)

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Dulbecco's phosphate buffered saline (DPBS), cell culture, MP Biomedicals

Dulbecco's phosphate buffered saline (DPBS), cell culture, MP Biomedicals

Supplier: MP Biomedicals

Phosphate buffered salts, Dulbecco's formula (DPBS) is a balanced salt solution (BSS) used for the handling and culturing of mammalian cells. DPBS is used to to irrigate, wash, and dilute mammalian cells.

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pH buffers, ready to use

pH buffers, ready to use

Supplier: WTW

Every bottle has its own lot reference with the expiration date.

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Buffer, HEPES solution (1 mol/L)

Supplier: EUROCLONE

HEPES buffer solution (1 mol/l)

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ISE sample conditioning buffers and solutions

Supplier: WTW

Ion strength adjustment solutions have multiple tasks: TISAB for fluoride measurements does not only adjust the pH value, by also decomplexes the fluoride-containing complex compounds. Other ion strength adjustment solutions ensure an adapted pH value as well as the addition of a suitable conducting salt to correctly capture the concentration.

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Protein extraction reagent, BugBuster® (primary amine-free), Millipore®

Supplier: Merck Millipore (Novagen)

BugBuster® (primary amine-free) Protein Extraction Reagent is a special formulation of BugBuster designed for applications where primary amines would interfere if present in the protein extract, such as protein immobilisation or cross-linking. The PIPPS buffer used in BugBuster® (primary amine-free) has a similar buffer capacity and pH range as the original Tris-buffered BugBuster®, but will not complex metal ions, also making it ideally suited for extraction of metal dependent proteins.

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Buffer, HEPES solution (1 mol/L)

Supplier: EUROCLONE

HEPES buffer solution (1 mol/l)

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Buffer solutions, colour coded, certified (20 °C), Fluka™

Supplier: Honeywell Chemicals

Borate, pH value: 9.00, 8.992 - 9.008, Reference temperature: 20 °C, Yellow

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Protein extraction reagent, BugBuster® 10X, Millipore®

Supplier: Merck Millipore (Novagen)

BugBuster® 10X is a concentrated formulation of the proprietary detergents employed in BugBuster® without the addition of salts or buffer components. Concentrated BugBuster® provides a flexible alternative to the ready-to-use standard 1X BugBuster®, allowing user-defined dilution and addition of buffer components. BugBuster® 10X has all of the bioprocessing benefits of standard BugBuster® plus the freedom to control pH, reagent concentration, and buffer additives necessary for maximum extraction and activity of your target protein.

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T-PER™ Tissue Protein Extraction Reagent

T-PER™ Tissue Protein Extraction Reagent

Supplier: Thermo Fisher Scientific

Tissue Protein Extraction Reagent (T-PER ) enables mild extraction of total protein from tissue samples.

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Protein extraction reagents, BugBuster® and BugBuster® plus Benzonase®, Millipore®

Supplier: Merck Millipore (Novagen)

BugBuster® Protein Extraction reagent is formulated for the gentle disruption of the cell wall of E. coli, liberating soluble protein. It provides a simple, rapid, low-cost alternative to mechanical methods such as French Press or sonication for releasing expressed target protein in preparation for purification or other applications. The formulation utilises a detergent mix that is capable of cell wall perforation without denaturing soluble protein.

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Buffer solutions

Buffer solutions

Supplier: SI Analytics

Buffer solutions intended for extremely precise calibration.

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TISAB III solution (Total Ionic Strength Adjustment Buffer) for fluoride determinations

TISAB III solution (Total Ionic Strength Adjustment Buffer) for fluoride determinations

Supplier: Mettler - Toledo

TISAB III solution for fluoride determinations

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Hank's balanced salt solution, modified, MP Biomedicals

Supplier: MP Biomedicals

Cell culture buffer, Hank's balanced salt solution, modified (1X)

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Protein extraction reagent, BugBuster® HT, Millipore®

Supplier: Merck Millipore (Novagen)

BugBuster® HT Protein Extraction Reagent combines BugBuster® Protein Extraction Reagent and Benzonase® Nuclease in one convenient reagent. BugBuster® HT eliminates common bioprocessing problems resulting from traditional cell lysis procedures. Soluble proteins are gently extracted from E. coli without exposure to heat or oxidative damage and viscosity is eliminated by nucleic acid digestion in a single step. The resulting protein extract can easily be fractionated by conventional purification techniques. BugBuster HT is ideally suited for application in high throughput protein purifications.

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Tris Hydrochloride

Supplier: Merck Millipore (Calbiochem‎)

Use of tetraethylammonium (TEA) and Tris loading for blocking TRPM7 channels in intact cells.: This study highlights the application of TRIS Hydrochloride in blocking TRPM7 channels, which is significant for cell signaling and ion channel regulation in pharmacological research (Holderby & Kozak, 2024).

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Buffer solutions

Buffer solutions

Supplier: REAGECON

Buffer solution, pH value: 4,00, ±0,01, Reference temperature: 20 °C, Plastic bottle

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Hank's balanced salts, MP Biomedicals

Supplier: MP Biomedicals

Hanks' balanced salts solution is used to maintain the pH and osmotic balance in the medium and to provide the cells with water and essential inorganic ions.

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Hanks' Balanced Salts (Modified), 1X, without Magnesium and Calcium, MP Biomedicals

Hanks' Balanced Salts (Modified), 1X, without Magnesium and Calcium, MP Biomedicals

Supplier: MP Biomedicals

Used to minimise cell attachment or aggregation.

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pH electrode calibration and maintenance solutions kit

pH electrode calibration and maintenance solutions kit

Supplier: WTW

Adapted to different electrode types for calibration and maintenance.

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pH buffer solutions, colour coded

Supplier: REAGECON

Colour coded pH buffer solutions for the calibration of pH meters.

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